Cargando…
Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide
In this study, a proper and reliable fluorometric method is introduced for screening acetylcholinesterase (AChE) and its inhibitors, using carbon quantum dots (CQDs) as the signal reporter. Pure, S-doped, and P-doped CQDs, were synthesized and their recoverable fluorescence quenching properties were...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558800/ https://www.ncbi.nlm.nih.gov/pubmed/37809678 http://dx.doi.org/10.1016/j.heliyon.2023.e19551 |
_version_ | 1785117360990978048 |
---|---|
author | Mahmoudi, Niloofar Fatemi, Fataneh Rahmandoust, Moones Mirzajani, Fateme Ranaei Siadat, Seyed Omid |
author_facet | Mahmoudi, Niloofar Fatemi, Fataneh Rahmandoust, Moones Mirzajani, Fateme Ranaei Siadat, Seyed Omid |
author_sort | Mahmoudi, Niloofar |
collection | PubMed |
description | In this study, a proper and reliable fluorometric method is introduced for screening acetylcholinesterase (AChE) and its inhibitors, using carbon quantum dots (CQDs) as the signal reporter. Pure, S-doped, and P-doped CQDs, were synthesized and their recoverable fluorescence quenching properties were observed, when exposed to Hg(2+), Cu(2+), and Fe(3+) quenching ions, respectively. The study on the recovery of their emission showed that after the introduction of another guest substance with a stronger affinity to the quenching ions, their fluorescence is restored. The Design Expert software was employed to compare the performance of the three CQDs, as fluorescent probes, based on their quenching efficiency and the percentage of their emission recovery in the presence of AChE and acetylthiocholine (ATCh). Based on the statistical analysis, among the studied CQDs, S-doped CQD was the most suitable candidate for sensor designing. The detection mechanism for the proposed S-doped CQD-based sensor is as follows: The strong binding of Cu(2+) ions to carboxyl groups of S-doped CQD quenches the fluorescence signal. Then, hydrolysis of ATCh into thiocholine (TCh) in the presence of AChE causes fluorescence recovery, due to the stronger affinity of Cu(2+) to the TCh, rather than the CQD. Finally, in the presence of malathion and chlorpyrifos inhibitors, AChE loses its ability to hydrolyze ATCh to TCh, so the fluorescence emission remains quenched. Based on the proposed detection technique, the designed sensor showed detection limits of 1.70 ppb and 1.50 ppb for malathion and chlorpyrifos, respectively. |
format | Online Article Text |
id | pubmed-10558800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105588002023-10-08 Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide Mahmoudi, Niloofar Fatemi, Fataneh Rahmandoust, Moones Mirzajani, Fateme Ranaei Siadat, Seyed Omid Heliyon Research Article In this study, a proper and reliable fluorometric method is introduced for screening acetylcholinesterase (AChE) and its inhibitors, using carbon quantum dots (CQDs) as the signal reporter. Pure, S-doped, and P-doped CQDs, were synthesized and their recoverable fluorescence quenching properties were observed, when exposed to Hg(2+), Cu(2+), and Fe(3+) quenching ions, respectively. The study on the recovery of their emission showed that after the introduction of another guest substance with a stronger affinity to the quenching ions, their fluorescence is restored. The Design Expert software was employed to compare the performance of the three CQDs, as fluorescent probes, based on their quenching efficiency and the percentage of their emission recovery in the presence of AChE and acetylthiocholine (ATCh). Based on the statistical analysis, among the studied CQDs, S-doped CQD was the most suitable candidate for sensor designing. The detection mechanism for the proposed S-doped CQD-based sensor is as follows: The strong binding of Cu(2+) ions to carboxyl groups of S-doped CQD quenches the fluorescence signal. Then, hydrolysis of ATCh into thiocholine (TCh) in the presence of AChE causes fluorescence recovery, due to the stronger affinity of Cu(2+) to the TCh, rather than the CQD. Finally, in the presence of malathion and chlorpyrifos inhibitors, AChE loses its ability to hydrolyze ATCh to TCh, so the fluorescence emission remains quenched. Based on the proposed detection technique, the designed sensor showed detection limits of 1.70 ppb and 1.50 ppb for malathion and chlorpyrifos, respectively. Elsevier 2023-08-30 /pmc/articles/PMC10558800/ /pubmed/37809678 http://dx.doi.org/10.1016/j.heliyon.2023.e19551 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Mahmoudi, Niloofar Fatemi, Fataneh Rahmandoust, Moones Mirzajani, Fateme Ranaei Siadat, Seyed Omid Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title | Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title_full | Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title_fullStr | Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title_full_unstemmed | Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title_short | Development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
title_sort | development of a carbon quantum dot-based sensor for the detection of acetylcholinesterase and the organophosphate pesticide |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558800/ https://www.ncbi.nlm.nih.gov/pubmed/37809678 http://dx.doi.org/10.1016/j.heliyon.2023.e19551 |
work_keys_str_mv | AT mahmoudiniloofar developmentofacarbonquantumdotbasedsensorforthedetectionofacetylcholinesteraseandtheorganophosphatepesticide AT fatemifataneh developmentofacarbonquantumdotbasedsensorforthedetectionofacetylcholinesteraseandtheorganophosphatepesticide AT rahmandoustmoones developmentofacarbonquantumdotbasedsensorforthedetectionofacetylcholinesteraseandtheorganophosphatepesticide AT mirzajanifateme developmentofacarbonquantumdotbasedsensorforthedetectionofacetylcholinesteraseandtheorganophosphatepesticide AT ranaeisiadatseyedomid developmentofacarbonquantumdotbasedsensorforthedetectionofacetylcholinesteraseandtheorganophosphatepesticide |