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Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis

The Pleurotus ostreatus mushroom, commonly referred to as the oyster mushroom, is a widely consumed edible mushroom that grows in clusters on dead or dying trees. In addition to its culinary uses, research has found that the mushroom may also have potential medicinal properties. The current study in...

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Autores principales: Alotaibi, Bader S., Malak, Nosheen, Khan, Afshan, Nasreen, Nasreen, Niaz, Sadaf, Ahmad, Imtiaz, Khan, Adil, Rodriguez-Vivas, Roger I., Chen, Chien-Chin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558824/
https://www.ncbi.nlm.nih.gov/pubmed/37809801
http://dx.doi.org/10.1016/j.heliyon.2023.e19600
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author Alotaibi, Bader S.
Malak, Nosheen
Khan, Afshan
Nasreen, Nasreen
Niaz, Sadaf
Ahmad, Imtiaz
Khan, Adil
Rodriguez-Vivas, Roger I.
Chen, Chien-Chin
author_facet Alotaibi, Bader S.
Malak, Nosheen
Khan, Afshan
Nasreen, Nasreen
Niaz, Sadaf
Ahmad, Imtiaz
Khan, Adil
Rodriguez-Vivas, Roger I.
Chen, Chien-Chin
author_sort Alotaibi, Bader S.
collection PubMed
description The Pleurotus ostreatus mushroom, commonly referred to as the oyster mushroom, is a widely consumed edible mushroom that grows in clusters on dead or dying trees. In addition to its culinary uses, research has found that the mushroom may also have potential medicinal properties. The current study investigated the potential use of a fungal extract from P. ostreatus as a natural acaricide against Rhipicephalus microplus, a major vector of economically significant infections and one of the most significant bovine ectoparasites. The study used the adult immersion test (AIT) and the larvae packet test (LPT) to evaluate the effectiveness of the extract against ticks. To evaluate the reproductive effect of the fungal extract on the reproduction of R. microplus engorged females, the eggs were tested for weight and egg-laying index. The results of the study showed that P. ostreatus extract had a significant acaricidal effect, with a 40 mg/mL concentration causing 89 ± 2.64% mortality in R. microplus larvae compared to the 91.00 ± 3.60% mortality by the positive control at 48 h interval. Whereas in the AIT, the extract inhibited 39.86 ± 4.13% of oviposition. The study also used in silico approach to further examine the binding mechanisms of the compounds in the fungal extract to the target protein R. microplus Triosephosphate isomerase RmTIM, using molecular docking in AutoDock Vina software. Docking scores of −8.3, −7.7 and −6.9 kcal/mol, respectively, showed that rutin, naringin and myricitin had significant interactions with the active site residues of the target protein. Our results suggest that P. ostreatus extract may be a potential alternative to traditional acaricides for controlling R. microplus in livestock.
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spelling pubmed-105588242023-10-08 Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis Alotaibi, Bader S. Malak, Nosheen Khan, Afshan Nasreen, Nasreen Niaz, Sadaf Ahmad, Imtiaz Khan, Adil Rodriguez-Vivas, Roger I. Chen, Chien-Chin Heliyon Research Article The Pleurotus ostreatus mushroom, commonly referred to as the oyster mushroom, is a widely consumed edible mushroom that grows in clusters on dead or dying trees. In addition to its culinary uses, research has found that the mushroom may also have potential medicinal properties. The current study investigated the potential use of a fungal extract from P. ostreatus as a natural acaricide against Rhipicephalus microplus, a major vector of economically significant infections and one of the most significant bovine ectoparasites. The study used the adult immersion test (AIT) and the larvae packet test (LPT) to evaluate the effectiveness of the extract against ticks. To evaluate the reproductive effect of the fungal extract on the reproduction of R. microplus engorged females, the eggs were tested for weight and egg-laying index. The results of the study showed that P. ostreatus extract had a significant acaricidal effect, with a 40 mg/mL concentration causing 89 ± 2.64% mortality in R. microplus larvae compared to the 91.00 ± 3.60% mortality by the positive control at 48 h interval. Whereas in the AIT, the extract inhibited 39.86 ± 4.13% of oviposition. The study also used in silico approach to further examine the binding mechanisms of the compounds in the fungal extract to the target protein R. microplus Triosephosphate isomerase RmTIM, using molecular docking in AutoDock Vina software. Docking scores of −8.3, −7.7 and −6.9 kcal/mol, respectively, showed that rutin, naringin and myricitin had significant interactions with the active site residues of the target protein. Our results suggest that P. ostreatus extract may be a potential alternative to traditional acaricides for controlling R. microplus in livestock. Elsevier 2023-08-29 /pmc/articles/PMC10558824/ /pubmed/37809801 http://dx.doi.org/10.1016/j.heliyon.2023.e19600 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Alotaibi, Bader S.
Malak, Nosheen
Khan, Afshan
Nasreen, Nasreen
Niaz, Sadaf
Ahmad, Imtiaz
Khan, Adil
Rodriguez-Vivas, Roger I.
Chen, Chien-Chin
Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title_full Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title_fullStr Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title_full_unstemmed Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title_short Acaricidal assessment of the fungal extract of Pleurotus ostreatus against Rhipicephalus microplus: Role of in vitro and in silico analysis
title_sort acaricidal assessment of the fungal extract of pleurotus ostreatus against rhipicephalus microplus: role of in vitro and in silico analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558824/
https://www.ncbi.nlm.nih.gov/pubmed/37809801
http://dx.doi.org/10.1016/j.heliyon.2023.e19600
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