Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes

Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles st...

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Detalles Bibliográficos
Autores principales: Pitre, Zephyr, Seltzer, Tess, Kalthoff, Cecilia, Benitez-Cortez, Maria, Yee, Conrad, Glennon, Elizabeth K.K., Primavera, Veronica I., Kaushansky, Alexis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558827/
https://www.ncbi.nlm.nih.gov/pubmed/37776521
http://dx.doi.org/10.1016/j.xpro.2023.102621
Descripción
Sumario:Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes. We describe steps for assembling a pupation station, self-emptying bucket, pupal funnel and dish vacuum, automatic aspirator, and sugar tubes. We also detail the application of these systems, along with specific limitations.

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