Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes

Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles st...

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Autores principales: Pitre, Zephyr, Seltzer, Tess, Kalthoff, Cecilia, Benitez-Cortez, Maria, Yee, Conrad, Glennon, Elizabeth K.K., Primavera, Veronica I., Kaushansky, Alexis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558827/
https://www.ncbi.nlm.nih.gov/pubmed/37776521
http://dx.doi.org/10.1016/j.xpro.2023.102621
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author Pitre, Zephyr
Seltzer, Tess
Kalthoff, Cecilia
Benitez-Cortez, Maria
Yee, Conrad
Glennon, Elizabeth K.K.
Primavera, Veronica I.
Kaushansky, Alexis
author_facet Pitre, Zephyr
Seltzer, Tess
Kalthoff, Cecilia
Benitez-Cortez, Maria
Yee, Conrad
Glennon, Elizabeth K.K.
Primavera, Veronica I.
Kaushansky, Alexis
author_sort Pitre, Zephyr
collection PubMed
description Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes. We describe steps for assembling a pupation station, self-emptying bucket, pupal funnel and dish vacuum, automatic aspirator, and sugar tubes. We also detail the application of these systems, along with specific limitations.
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spelling pubmed-105588272023-10-08 Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes Pitre, Zephyr Seltzer, Tess Kalthoff, Cecilia Benitez-Cortez, Maria Yee, Conrad Glennon, Elizabeth K.K. Primavera, Veronica I. Kaushansky, Alexis STAR Protoc Protocol Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes. We describe steps for assembling a pupation station, self-emptying bucket, pupal funnel and dish vacuum, automatic aspirator, and sugar tubes. We also detail the application of these systems, along with specific limitations. Elsevier 2023-09-28 /pmc/articles/PMC10558827/ /pubmed/37776521 http://dx.doi.org/10.1016/j.xpro.2023.102621 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pitre, Zephyr
Seltzer, Tess
Kalthoff, Cecilia
Benitez-Cortez, Maria
Yee, Conrad
Glennon, Elizabeth K.K.
Primavera, Veronica I.
Kaushansky, Alexis
Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title_full Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title_fullStr Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title_full_unstemmed Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title_short Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes
title_sort protocol for assembling and implementing a partially automated system for rearing and handling anopheles stephensi mosquitoes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558827/
https://www.ncbi.nlm.nih.gov/pubmed/37776521
http://dx.doi.org/10.1016/j.xpro.2023.102621
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