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Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infection
The lack of physiologically relevant in vitro models has hampered progress in understanding human lung development and disease. Here, we describe a protocol in which human induced pluripotent stem cells (hiPSCs) undergo stepwise differentiation into definitive endoderm (>88% population) to three-...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558843/ https://www.ncbi.nlm.nih.gov/pubmed/37809493 http://dx.doi.org/10.1016/j.heliyon.2023.e19601 |