Bacteria-derived DNA in serum extracellular vesicles are biomarkers for renal cell carcinoma

This is the first study to determine the clinical importance of circulating bacterial DNA in patients with renal cell carcinoma (RCC). We performed 16S rRNA metagenomic analysis of serum extracellular vesicles (EVs) from 88 patients with RCC and 10 healthy donors and identified three abundant bacter...

Descripción completa

Detalles Bibliográficos
Autores principales: Uemura, Toshihiro, Kawashima, Atsunari, Jingushi, Kentaro, Motooka, Daisuke, Saito, Takuro, Nesrine, Sassi, Oka, Toshiki, Okuda, Yohei, Yamamoto, Akinaru, Yamamichi, Gaku, Tomiyama, Eisuke, Ishizuya, Yu, Yamamoto, Yoshiyuki, Kato, Taigo, Hatano, Koji, Tsujikawa, Kazutake, Wada, Hisashi, Nonomura, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559165/
https://www.ncbi.nlm.nih.gov/pubmed/37810127
http://dx.doi.org/10.1016/j.heliyon.2023.e19800
Descripción
Sumario:This is the first study to determine the clinical importance of circulating bacterial DNA in patients with renal cell carcinoma (RCC). We performed 16S rRNA metagenomic analysis of serum extracellular vesicles (EVs) from 88 patients with RCC and 10 healthy donors and identified three abundant bacterial DNA: Bacteroidia, TM7-1, and Sphingomonadales. Combining characteristic bacterial DNA information (three bacteria-derived DNA), a BTS index was created to diagnose patients with RCC. The BTS index showed high sensitivity not only in the discovery cohort, but also in the validation cohort, suggesting that it was useful as a screening test. Furthermore, in nivolumab treatment of RCC, patients with higher levels of Bacteroidia DNA in serum EVs had significantly poorer progression-free and overall survival than did those with lower levels. This study showed that circulating Bacteria-derived DNA could be used as a biomarker for RCC.

Ejemplares similares