Current approaches toward the removal of methylene blue dye from synthetic textile effluent using bacterial treated agricultural waste absorbent through statistical design

Massive amounts of wastewater are produced by the textile industry, and this waste needs to be appropriately managed. Agricultural waste wheat straw (WS), a biosorbent that is both economically available and environmentally acceptable, was used in this work to treat textile effluent. Microbial treated modification approaches were utilized for WS to study the dye removal from textile wastewater. Total 15 different isolates were screened for the dye degradation ability from Surat textile industrial effluent. The most significant deterioration was seen in PPSUHB3 when compared to other isolates. The amount of methylene blue dye removal was examined using the isolate PPSUHB3 due to its high efficiency. Based on 16s rDNA sequencing, it was predicted that the isolate PPSUHB3 was Bacillus licheniformis, having great capacity to degrade dye & wheat straw by producing efficient enzyme. The isolate showed the highest decolorization % of MB dye during optimization with WS absorbent which was verified using FTIR and SEM. The dye removal process parameters were statistically optimized using a central composite design (CCD). Wheat straw with particle sizes of 180–250 mm was discovered to be a possible adsorbent for the removal of colour. The maximum removal of MB (55.89%) was obtained using a statistical experimental design at pH 6.36, Temperature 44.6 °C, and Bacteria Concentration 3.04%. The created model is highly significant, according to the ANOVA, which found an R2 value of 0.9812 for it. The validation experiment revealed that the experimental and projected results were strikingly similar. The study found that using bacterial treated wheat straw as an adsorbent may remove wastewater that contains colours at a low cost.