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Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease

Understanding gut bacterial composition and proteome changes in patients with early-stage chronic kidney disease (CKD) could lead to better methods of controlling the disease progression. Here, we investigated the gut microbiome and microbial functions in patients with S. stercoralis infection (stro...

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Autores principales: Tran, Na T.D., Chaidee, Apisit, Surapinit, Achirawit, Yingklang, Manachai, Roytrakul, Sitiruk, Charoenlappanit, Sawanya, Pinlaor, Porntip, Hongsrichan, Nuttanan, Nguyen Thi, Hai, Anutrakulchai, Sirirat, Cha'on, Ubon, Pinlaor, Somchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559256/
https://www.ncbi.nlm.nih.gov/pubmed/37809389
http://dx.doi.org/10.1016/j.heliyon.2023.e19859
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author Tran, Na T.D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Nguyen Thi, Hai
Anutrakulchai, Sirirat
Cha'on, Ubon
Pinlaor, Somchai
author_facet Tran, Na T.D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Nguyen Thi, Hai
Anutrakulchai, Sirirat
Cha'on, Ubon
Pinlaor, Somchai
author_sort Tran, Na T.D.
collection PubMed
description Understanding gut bacterial composition and proteome changes in patients with early-stage chronic kidney disease (CKD) could lead to better methods of controlling the disease progression. Here, we investigated the gut microbiome and microbial functions in patients with S. stercoralis infection (strongyloidiasis) and early-stage CKD. Thirty-five patients with early stages (1–3) of CKD were placed in two groups matched for population characteristics and biochemical parameters, 12 patients with strongyloidiasis in one group and 23 uninfected patients in the other. From every individual, a sample of their feces was obtained and processed for 16S rRNA sequencing and metaproteomic analysis using tandem liquid chromatography-mass spectrometry (LC-MS/MS). Strongyloides stercoralis infection per se did not significantly alter gut microbial diversity. However, certain genera (Bacteroides, Faecalibacterium, Fusicatenibacter, Sarcina, and Anaerostipes) were significantly more abundant in infection-free CKD patients than in infected individuals. The genera Peptoclostridium and Catenibacterium were enriched in infected patients. Among the significantly altered genera, Fusicatenibacter and Anaerostipes were the most correlated with renal parameters. The relative abundance of members of the genus Fusicatenibacter was moderately positively correlated with estimated glomerular filtration rate (eGFR) (r = 0.335, p = 0.049) and negatively with serum creatinine (r = −0.35, p = 0.039). Anaerostipes, on the other hand, showed a near-significant positive correlation with eGFR (r = 0.296, p = 0.084). Individuals with S. stercoralis infection had higher levels of bacterial proteins involved in amino-acid metabolism. Analysis using STITCH predicted that bacterial amino-acid metabolism may also be involved in the production of colon-derived uremic toxin (indole), a toxic substance known to promote CKD. Strongyloides stercoralis infection is, therefore, associated with reduced abundance of Fusicatenibacter and Anaerostipes (two genera possibly beneficial for kidney function) and with increased bacterial amino-acid metabolism in the early-stages of CKD, potentially producing uremic toxin. This study provides useful information for prevention of progression of CKD beyond the early stages.
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spelling pubmed-105592562023-10-08 Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease Tran, Na T.D. Chaidee, Apisit Surapinit, Achirawit Yingklang, Manachai Roytrakul, Sitiruk Charoenlappanit, Sawanya Pinlaor, Porntip Hongsrichan, Nuttanan Nguyen Thi, Hai Anutrakulchai, Sirirat Cha'on, Ubon Pinlaor, Somchai Heliyon Research Article Understanding gut bacterial composition and proteome changes in patients with early-stage chronic kidney disease (CKD) could lead to better methods of controlling the disease progression. Here, we investigated the gut microbiome and microbial functions in patients with S. stercoralis infection (strongyloidiasis) and early-stage CKD. Thirty-five patients with early stages (1–3) of CKD were placed in two groups matched for population characteristics and biochemical parameters, 12 patients with strongyloidiasis in one group and 23 uninfected patients in the other. From every individual, a sample of their feces was obtained and processed for 16S rRNA sequencing and metaproteomic analysis using tandem liquid chromatography-mass spectrometry (LC-MS/MS). Strongyloides stercoralis infection per se did not significantly alter gut microbial diversity. However, certain genera (Bacteroides, Faecalibacterium, Fusicatenibacter, Sarcina, and Anaerostipes) were significantly more abundant in infection-free CKD patients than in infected individuals. The genera Peptoclostridium and Catenibacterium were enriched in infected patients. Among the significantly altered genera, Fusicatenibacter and Anaerostipes were the most correlated with renal parameters. The relative abundance of members of the genus Fusicatenibacter was moderately positively correlated with estimated glomerular filtration rate (eGFR) (r = 0.335, p = 0.049) and negatively with serum creatinine (r = −0.35, p = 0.039). Anaerostipes, on the other hand, showed a near-significant positive correlation with eGFR (r = 0.296, p = 0.084). Individuals with S. stercoralis infection had higher levels of bacterial proteins involved in amino-acid metabolism. Analysis using STITCH predicted that bacterial amino-acid metabolism may also be involved in the production of colon-derived uremic toxin (indole), a toxic substance known to promote CKD. Strongyloides stercoralis infection is, therefore, associated with reduced abundance of Fusicatenibacter and Anaerostipes (two genera possibly beneficial for kidney function) and with increased bacterial amino-acid metabolism in the early-stages of CKD, potentially producing uremic toxin. This study provides useful information for prevention of progression of CKD beyond the early stages. Elsevier 2023-09-06 /pmc/articles/PMC10559256/ /pubmed/37809389 http://dx.doi.org/10.1016/j.heliyon.2023.e19859 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Tran, Na T.D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Nguyen Thi, Hai
Anutrakulchai, Sirirat
Cha'on, Ubon
Pinlaor, Somchai
Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title_full Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title_fullStr Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title_full_unstemmed Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title_short Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
title_sort strongyloides stercoralis infection reduces fusicatenibacter and anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559256/
https://www.ncbi.nlm.nih.gov/pubmed/37809389
http://dx.doi.org/10.1016/j.heliyon.2023.e19859
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