A review of protocols for engineering human cardiac organoids

The use of human cardiac organoids (hCOs) as 3D in vitro models for cardiovascular research has shown great promise. Human pluripotent stem cells (hPSCs) have proven to be a potent source for engineering hCOs. However, various protocols for generating hCOs from hPSCs result in significant difference...

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Detalles Bibliográficos
Autores principales: Wu, Fujian, He, Qian, Li, Furong, Yang, Xiaofei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559357/
https://www.ncbi.nlm.nih.gov/pubmed/37809996
http://dx.doi.org/10.1016/j.heliyon.2023.e19938
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author Wu, Fujian
He, Qian
Li, Furong
Yang, Xiaofei
author_facet Wu, Fujian
He, Qian
Li, Furong
Yang, Xiaofei
author_sort Wu, Fujian
collection PubMed
description The use of human cardiac organoids (hCOs) as 3D in vitro models for cardiovascular research has shown great promise. Human pluripotent stem cells (hPSCs) have proven to be a potent source for engineering hCOs. However, various protocols for generating hCOs from hPSCs result in significant differences in heart development, maturity, complexity, vascularization, and spatial structure, all of which can influence their functional and physiological properties. This protocol review aims to highlight different strategies for generating hCOs using hPSCs while also critically discussing their challenges and limitations.
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spelling pubmed-105593572023-10-08 A review of protocols for engineering human cardiac organoids Wu, Fujian He, Qian Li, Furong Yang, Xiaofei Heliyon Review Article The use of human cardiac organoids (hCOs) as 3D in vitro models for cardiovascular research has shown great promise. Human pluripotent stem cells (hPSCs) have proven to be a potent source for engineering hCOs. However, various protocols for generating hCOs from hPSCs result in significant differences in heart development, maturity, complexity, vascularization, and spatial structure, all of which can influence their functional and physiological properties. This protocol review aims to highlight different strategies for generating hCOs using hPSCs while also critically discussing their challenges and limitations. Elsevier 2023-09-07 /pmc/articles/PMC10559357/ /pubmed/37809996 http://dx.doi.org/10.1016/j.heliyon.2023.e19938 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Wu, Fujian
He, Qian
Li, Furong
Yang, Xiaofei
A review of protocols for engineering human cardiac organoids
title A review of protocols for engineering human cardiac organoids
title_full A review of protocols for engineering human cardiac organoids
title_fullStr A review of protocols for engineering human cardiac organoids
title_full_unstemmed A review of protocols for engineering human cardiac organoids
title_short A review of protocols for engineering human cardiac organoids
title_sort review of protocols for engineering human cardiac organoids
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559357/
https://www.ncbi.nlm.nih.gov/pubmed/37809996
http://dx.doi.org/10.1016/j.heliyon.2023.e19938
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