Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay

BACKGROUND: Bacillus subtilis is one of the workhorses in industrial biotechnology and well known for its secretion potential. Efficient secretion of recombinant proteins still requires extensive optimization campaigns and screening with activity-based methods. However, not every protein can be dete...

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Detalles Bibliográficos
Autores principales: Lenz, Patrick, Bakkes, Patrick J., Müller, Carolin, Malek, Marzena, Freudl, Roland, Oldiges, Marco, Drepper, Thomas, Jaeger, Karl-Erich, Knapp, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559633/
https://www.ncbi.nlm.nih.gov/pubmed/37805580
http://dx.doi.org/10.1186/s12934-023-02199-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10559633/
https://www.ncbi.nlm.nih.gov/pubmed/37805580
http://dx.doi.org/10.1186/s12934-023-02199-8

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