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Design and construction of multi epitope- peptide vaccine candidate for rabies virus

Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that t...

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Autores principales: Abdulhameed Odhar, Hasanain, Hashim, Ahmed Fadhil, Humadi, Suhad Sami, Ahjel, Salam Waheed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560302/
https://www.ncbi.nlm.nih.gov/pubmed/37814687
http://dx.doi.org/10.6026/97320630019167
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author Abdulhameed Odhar, Hasanain
Hashim, Ahmed Fadhil
Humadi, Suhad Sami
Ahjel, Salam Waheed
author_facet Abdulhameed Odhar, Hasanain
Hashim, Ahmed Fadhil
Humadi, Suhad Sami
Ahjel, Salam Waheed
author_sort Abdulhameed Odhar, Hasanain
collection PubMed
description Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that the entry of rabies virus into host cells is mediated by a trimeric glycoprotein presents on the surface of viral envelope. As the sole viral surface protein, this trimeric glycoprotein represents a promising molecular target to design new vaccines and neutralizing antibodies against rabies virus. Epitope mapping studies had identified several antigenic sites on the surface of trimeric pre-fusion glycoprotein of rabies virus. Therefore, it is of interest to screen the rabies virus glycoprotein by different web-based immuno-informatics tools to identify potential B-cells and T-cells linear epitopes. Here, we present a construct of peptide vaccine that consists of these predicted linear epitopes of rabies virus glycoprotein together with appropriate linkers and adjuvant. Various online prediction tools, molecular docking and dynamics simulation assume that the vaccine construct may be stable, safe and effective. However, validation of these in-silico results is necessary both in vitro and in vivo setting.
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spelling pubmed-105603022023-10-09 Design and construction of multi epitope- peptide vaccine candidate for rabies virus Abdulhameed Odhar, Hasanain Hashim, Ahmed Fadhil Humadi, Suhad Sami Ahjel, Salam Waheed Bioinformation Research Article Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that the entry of rabies virus into host cells is mediated by a trimeric glycoprotein presents on the surface of viral envelope. As the sole viral surface protein, this trimeric glycoprotein represents a promising molecular target to design new vaccines and neutralizing antibodies against rabies virus. Epitope mapping studies had identified several antigenic sites on the surface of trimeric pre-fusion glycoprotein of rabies virus. Therefore, it is of interest to screen the rabies virus glycoprotein by different web-based immuno-informatics tools to identify potential B-cells and T-cells linear epitopes. Here, we present a construct of peptide vaccine that consists of these predicted linear epitopes of rabies virus glycoprotein together with appropriate linkers and adjuvant. Various online prediction tools, molecular docking and dynamics simulation assume that the vaccine construct may be stable, safe and effective. However, validation of these in-silico results is necessary both in vitro and in vivo setting. Biomedical Informatics 2023-02-28 /pmc/articles/PMC10560302/ /pubmed/37814687 http://dx.doi.org/10.6026/97320630019167 Text en © 2023 Biomedical Informatics https://creativecommons.org/licenses/by/3.0/This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Abdulhameed Odhar, Hasanain
Hashim, Ahmed Fadhil
Humadi, Suhad Sami
Ahjel, Salam Waheed
Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title_full Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title_fullStr Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title_full_unstemmed Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title_short Design and construction of multi epitope- peptide vaccine candidate for rabies virus
title_sort design and construction of multi epitope- peptide vaccine candidate for rabies virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560302/
https://www.ncbi.nlm.nih.gov/pubmed/37814687
http://dx.doi.org/10.6026/97320630019167
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