Cargando…
Design and construction of multi epitope- peptide vaccine candidate for rabies virus
Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that t...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Biomedical Informatics
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560302/ https://www.ncbi.nlm.nih.gov/pubmed/37814687 http://dx.doi.org/10.6026/97320630019167 |
_version_ | 1785117703962361856 |
---|---|
author | Abdulhameed Odhar, Hasanain Hashim, Ahmed Fadhil Humadi, Suhad Sami Ahjel, Salam Waheed |
author_facet | Abdulhameed Odhar, Hasanain Hashim, Ahmed Fadhil Humadi, Suhad Sami Ahjel, Salam Waheed |
author_sort | Abdulhameed Odhar, Hasanain |
collection | PubMed |
description | Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that the entry of rabies virus into host cells is mediated by a trimeric glycoprotein presents on the surface of viral envelope. As the sole viral surface protein, this trimeric glycoprotein represents a promising molecular target to design new vaccines and neutralizing antibodies against rabies virus. Epitope mapping studies had identified several antigenic sites on the surface of trimeric pre-fusion glycoprotein of rabies virus. Therefore, it is of interest to screen the rabies virus glycoprotein by different web-based immuno-informatics tools to identify potential B-cells and T-cells linear epitopes. Here, we present a construct of peptide vaccine that consists of these predicted linear epitopes of rabies virus glycoprotein together with appropriate linkers and adjuvant. Various online prediction tools, molecular docking and dynamics simulation assume that the vaccine construct may be stable, safe and effective. However, validation of these in-silico results is necessary both in vitro and in vivo setting. |
format | Online Article Text |
id | pubmed-10560302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Biomedical Informatics |
record_format | MEDLINE/PubMed |
spelling | pubmed-105603022023-10-09 Design and construction of multi epitope- peptide vaccine candidate for rabies virus Abdulhameed Odhar, Hasanain Hashim, Ahmed Fadhil Humadi, Suhad Sami Ahjel, Salam Waheed Bioinformation Research Article Rabies virus is a zoonotic pathogen that causes lethal encephalitis with a case fatality rate of almost 100% in unvaccinated individuals. The currently available vaccines against rabies are composed of inactivated viral particles that only confer a short-term immune response. It is well-known that the entry of rabies virus into host cells is mediated by a trimeric glycoprotein presents on the surface of viral envelope. As the sole viral surface protein, this trimeric glycoprotein represents a promising molecular target to design new vaccines and neutralizing antibodies against rabies virus. Epitope mapping studies had identified several antigenic sites on the surface of trimeric pre-fusion glycoprotein of rabies virus. Therefore, it is of interest to screen the rabies virus glycoprotein by different web-based immuno-informatics tools to identify potential B-cells and T-cells linear epitopes. Here, we present a construct of peptide vaccine that consists of these predicted linear epitopes of rabies virus glycoprotein together with appropriate linkers and adjuvant. Various online prediction tools, molecular docking and dynamics simulation assume that the vaccine construct may be stable, safe and effective. However, validation of these in-silico results is necessary both in vitro and in vivo setting. Biomedical Informatics 2023-02-28 /pmc/articles/PMC10560302/ /pubmed/37814687 http://dx.doi.org/10.6026/97320630019167 Text en © 2023 Biomedical Informatics https://creativecommons.org/licenses/by/3.0/This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Research Article Abdulhameed Odhar, Hasanain Hashim, Ahmed Fadhil Humadi, Suhad Sami Ahjel, Salam Waheed Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title | Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title_full | Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title_fullStr | Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title_full_unstemmed | Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title_short | Design and construction of multi epitope- peptide vaccine candidate for rabies virus |
title_sort | design and construction of multi epitope- peptide vaccine candidate for rabies virus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560302/ https://www.ncbi.nlm.nih.gov/pubmed/37814687 http://dx.doi.org/10.6026/97320630019167 |
work_keys_str_mv | AT abdulhameedodharhasanain designandconstructionofmultiepitopepeptidevaccinecandidateforrabiesvirus AT hashimahmedfadhil designandconstructionofmultiepitopepeptidevaccinecandidateforrabiesvirus AT humadisuhadsami designandconstructionofmultiepitopepeptidevaccinecandidateforrabiesvirus AT ahjelsalamwaheed designandconstructionofmultiepitopepeptidevaccinecandidateforrabiesvirus |