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Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans

Agaro-oligosaccharides (AOSs) are known to have biological activities, such as anti-inflammatory, anti-tumor, and anti-obesity effects. Although existing evidence suggests the presence of AOSs in peripheral tissues after oral administration, whether AOSs permeate into the blood circulation remains u...

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Autores principales: Shirai, Ikuya, Iwasaki, Yu, Karasawa, Koji, Shigemura, Yasutaka, Katayama, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560721/
https://www.ncbi.nlm.nih.gov/pubmed/37818337
http://dx.doi.org/10.3389/fnut.2023.1273328
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author Shirai, Ikuya
Iwasaki, Yu
Karasawa, Koji
Shigemura, Yasutaka
Katayama, Shigeru
author_facet Shirai, Ikuya
Iwasaki, Yu
Karasawa, Koji
Shigemura, Yasutaka
Katayama, Shigeru
author_sort Shirai, Ikuya
collection PubMed
description Agaro-oligosaccharides (AOSs) are known to have biological activities, such as anti-inflammatory, anti-tumor, and anti-obesity effects. Although existing evidence suggests the presence of AOSs in peripheral tissues after oral administration, whether AOSs permeate into the blood circulation remains unknown. Thus, we hypothesized that AOSs with low-molecular weight can permeate the human gastrointestinal tract. To test this hypothesis, the time course of absorption was examined by analyzing plasma samples before and 1, 2, and 4 h after ingestion. Analysis was performed using liquid chromatography/mass spectrometry after labeling with p-aminobenzoic ethyl ester. Our results showed that the plasma concentration of agarobiose (Abi) was higher than that of agarotetraose (Ate); however, agarohexaose was not detected. Additionally, plasma levels of Abi and Ate were proportional to the dose. These results suggest that permeation efficiency is dependent on the molecular weight and that the systemic absorption of Abi via the gastrointestinal tract is better than that of Ate. These findings will contribute to a better understanding of the bioactivity of orally administered AOSs in peripheral tissues.
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spelling pubmed-105607212023-10-10 Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans Shirai, Ikuya Iwasaki, Yu Karasawa, Koji Shigemura, Yasutaka Katayama, Shigeru Front Nutr Nutrition Agaro-oligosaccharides (AOSs) are known to have biological activities, such as anti-inflammatory, anti-tumor, and anti-obesity effects. Although existing evidence suggests the presence of AOSs in peripheral tissues after oral administration, whether AOSs permeate into the blood circulation remains unknown. Thus, we hypothesized that AOSs with low-molecular weight can permeate the human gastrointestinal tract. To test this hypothesis, the time course of absorption was examined by analyzing plasma samples before and 1, 2, and 4 h after ingestion. Analysis was performed using liquid chromatography/mass spectrometry after labeling with p-aminobenzoic ethyl ester. Our results showed that the plasma concentration of agarobiose (Abi) was higher than that of agarotetraose (Ate); however, agarohexaose was not detected. Additionally, plasma levels of Abi and Ate were proportional to the dose. These results suggest that permeation efficiency is dependent on the molecular weight and that the systemic absorption of Abi via the gastrointestinal tract is better than that of Ate. These findings will contribute to a better understanding of the bioactivity of orally administered AOSs in peripheral tissues. Frontiers Media S.A. 2023-09-25 /pmc/articles/PMC10560721/ /pubmed/37818337 http://dx.doi.org/10.3389/fnut.2023.1273328 Text en Copyright © 2023 Shirai, Iwasaki, Karasawa, Shigemura and Katayama. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Shirai, Ikuya
Iwasaki, Yu
Karasawa, Koji
Shigemura, Yasutaka
Katayama, Shigeru
Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title_full Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title_fullStr Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title_full_unstemmed Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title_short Orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
title_sort orally administered low-molecular weight agaro-oligosaccharides are absorbed into the plasma of healthy humans
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560721/
https://www.ncbi.nlm.nih.gov/pubmed/37818337
http://dx.doi.org/10.3389/fnut.2023.1273328
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