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Massively-multiplexed epitope mapping techniques for viral antigen discovery

Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites...

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Detalles Bibliográficos
Autores principales: Hu, Diya, Irving, Aaron T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561112/
https://www.ncbi.nlm.nih.gov/pubmed/37818363
http://dx.doi.org/10.3389/fimmu.2023.1192385
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author Hu, Diya
Irving, Aaron T.
author_facet Hu, Diya
Irving, Aaron T.
author_sort Hu, Diya
collection PubMed
description Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.
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spelling pubmed-105611122023-10-10 Massively-multiplexed epitope mapping techniques for viral antigen discovery Hu, Diya Irving, Aaron T. Front Immunol Immunology Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery. Frontiers Media S.A. 2023-09-25 /pmc/articles/PMC10561112/ /pubmed/37818363 http://dx.doi.org/10.3389/fimmu.2023.1192385 Text en Copyright © 2023 Hu and Irving https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hu, Diya
Irving, Aaron T.
Massively-multiplexed epitope mapping techniques for viral antigen discovery
title Massively-multiplexed epitope mapping techniques for viral antigen discovery
title_full Massively-multiplexed epitope mapping techniques for viral antigen discovery
title_fullStr Massively-multiplexed epitope mapping techniques for viral antigen discovery
title_full_unstemmed Massively-multiplexed epitope mapping techniques for viral antigen discovery
title_short Massively-multiplexed epitope mapping techniques for viral antigen discovery
title_sort massively-multiplexed epitope mapping techniques for viral antigen discovery
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561112/
https://www.ncbi.nlm.nih.gov/pubmed/37818363
http://dx.doi.org/10.3389/fimmu.2023.1192385
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