How to better select SARS‐CoV‐2 preservation solution of virus nucleic acid testing

BACKGROUND: Sampling and testing for SARS‐CoV‐2 is a widely recognized method for identifying patients with COVID‐19. However, there is limited research available on the stability of nucleic acids in viral storage solutions. METHODS: This paper investigates the components that provide better protection for virus and nucleic acid detection. The study utilized real‐time quantitative fluorescent PCR to detect SARS‐CoV‐2 and evaluate the preservation effect and stability of SARS‐CoV‐2 viral storage solution under various conditions, including different guanidinium salts, brands, and storage conditions. RESULTS: All brands of inactivated virus preservation solutions demonstrated effective preservation and stability. However, 0.5 mol/L guanidine hydrochloride and guanidine isothiocyanate solutions exhibited poor antiseptic effects. Additionally, refrigerated storage showed better preservation compared to room temperature storage. CONCLUSIONS: We recommend using inactivated virus collection solution to preserve and transport samples and testing preferably within 6 hours to reduce false negatives of NAT results.