Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma

The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was...

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Autores principales: Chakraborty, Rajdeep, Darido, Charbel, Chien, Arthur, Tay, Aidan, Vickery, Karen, Hu, Honghua, Liu, Fei, Ranganathan, Shoba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562364/
https://www.ncbi.nlm.nih.gov/pubmed/37813936
http://dx.doi.org/10.1038/s41598-023-44329-7
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author Chakraborty, Rajdeep
Darido, Charbel
Chien, Arthur
Tay, Aidan
Vickery, Karen
Hu, Honghua
Liu, Fei
Ranganathan, Shoba
author_facet Chakraborty, Rajdeep
Darido, Charbel
Chien, Arthur
Tay, Aidan
Vickery, Karen
Hu, Honghua
Liu, Fei
Ranganathan, Shoba
author_sort Chakraborty, Rajdeep
collection PubMed
description The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3(+)/IL-2Rα(+)/CD25(+)) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30(+)/IFN-γ(+)) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001).
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spelling pubmed-105623642023-10-11 Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma Chakraborty, Rajdeep Darido, Charbel Chien, Arthur Tay, Aidan Vickery, Karen Hu, Honghua Liu, Fei Ranganathan, Shoba Sci Rep Article The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3(+)/IL-2Rα(+)/CD25(+)) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30(+)/IFN-γ(+)) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001). Nature Publishing Group UK 2023-10-09 /pmc/articles/PMC10562364/ /pubmed/37813936 http://dx.doi.org/10.1038/s41598-023-44329-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chakraborty, Rajdeep
Darido, Charbel
Chien, Arthur
Tay, Aidan
Vickery, Karen
Hu, Honghua
Liu, Fei
Ranganathan, Shoba
Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title_full Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title_fullStr Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title_full_unstemmed Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title_short Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
title_sort preclinical 3d-model supports an invisibility cloak for adenoid cystic carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562364/
https://www.ncbi.nlm.nih.gov/pubmed/37813936
http://dx.doi.org/10.1038/s41598-023-44329-7
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