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Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity

The human protozoan parasite Entamoeba histolytica is responsible for amebiasis, a disease endemic to developing countries. E. histolytica trophozoites colonize the large intestine, primarily feeding on bacteria. However, in the gastrointestinal tract, bacterial cells form aggregates or structured c...

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Autores principales: Zanditenas, Eva, Trebicz-Geffen, Meirav, Kolli, Divya, Domínguez-García, Laura, Farhi, Einan, Linde, Liat, Romero, Diego, Chapman, Matthew, Kolodkin-Gal, Ilana, Ankri, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562373/
https://www.ncbi.nlm.nih.gov/pubmed/37813896
http://dx.doi.org/10.1038/s41522-023-00444-x
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author Zanditenas, Eva
Trebicz-Geffen, Meirav
Kolli, Divya
Domínguez-García, Laura
Farhi, Einan
Linde, Liat
Romero, Diego
Chapman, Matthew
Kolodkin-Gal, Ilana
Ankri, Serge
author_facet Zanditenas, Eva
Trebicz-Geffen, Meirav
Kolli, Divya
Domínguez-García, Laura
Farhi, Einan
Linde, Liat
Romero, Diego
Chapman, Matthew
Kolodkin-Gal, Ilana
Ankri, Serge
author_sort Zanditenas, Eva
collection PubMed
description The human protozoan parasite Entamoeba histolytica is responsible for amebiasis, a disease endemic to developing countries. E. histolytica trophozoites colonize the large intestine, primarily feeding on bacteria. However, in the gastrointestinal tract, bacterial cells form aggregates or structured communities called biofilms too large for phagocytosis. Remarkably, trophozoites are still able to invade and degrade established biofilms, utilizing a mechanism that mimics digestive exophagy. Digestive exophagy refers to the secretion of digestive enzymes that promote the digestion of objects too large for direct phagocytosis by phagocytes. E. histolytica cysteine proteinases (CPs) play a crucial role in the degradation process of Bacillus subtilis biofilm. These proteinases target TasA, a major component of the B. subtilis biofilm matrix, also contributing to the adhesion of the parasite to the biofilm. In addition, they are also involved in the degradation of biofilms formed by Gram-negative and Gram-positive enteric pathogens. Furthermore, biofilms also play an important role in protecting trophozoites against oxidative stress. This specific mechanism suggests that the amoeba has adapted to prey on biofilms, potentially serving as an untapped reservoir for novel therapeutic approaches to treat biofilms. Consistently, products derived from the amoeba have been shown to restore antibiotic sensitivity to biofilm cells. In addition, our findings reveal that probiotic biofilms can act as a protective shield for mammalian cells, hindering the progression of the parasite towards them.
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spelling pubmed-105623732023-10-11 Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity Zanditenas, Eva Trebicz-Geffen, Meirav Kolli, Divya Domínguez-García, Laura Farhi, Einan Linde, Liat Romero, Diego Chapman, Matthew Kolodkin-Gal, Ilana Ankri, Serge NPJ Biofilms Microbiomes Article The human protozoan parasite Entamoeba histolytica is responsible for amebiasis, a disease endemic to developing countries. E. histolytica trophozoites colonize the large intestine, primarily feeding on bacteria. However, in the gastrointestinal tract, bacterial cells form aggregates or structured communities called biofilms too large for phagocytosis. Remarkably, trophozoites are still able to invade and degrade established biofilms, utilizing a mechanism that mimics digestive exophagy. Digestive exophagy refers to the secretion of digestive enzymes that promote the digestion of objects too large for direct phagocytosis by phagocytes. E. histolytica cysteine proteinases (CPs) play a crucial role in the degradation process of Bacillus subtilis biofilm. These proteinases target TasA, a major component of the B. subtilis biofilm matrix, also contributing to the adhesion of the parasite to the biofilm. In addition, they are also involved in the degradation of biofilms formed by Gram-negative and Gram-positive enteric pathogens. Furthermore, biofilms also play an important role in protecting trophozoites against oxidative stress. This specific mechanism suggests that the amoeba has adapted to prey on biofilms, potentially serving as an untapped reservoir for novel therapeutic approaches to treat biofilms. Consistently, products derived from the amoeba have been shown to restore antibiotic sensitivity to biofilm cells. In addition, our findings reveal that probiotic biofilms can act as a protective shield for mammalian cells, hindering the progression of the parasite towards them. Nature Publishing Group UK 2023-10-09 /pmc/articles/PMC10562373/ /pubmed/37813896 http://dx.doi.org/10.1038/s41522-023-00444-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zanditenas, Eva
Trebicz-Geffen, Meirav
Kolli, Divya
Domínguez-García, Laura
Farhi, Einan
Linde, Liat
Romero, Diego
Chapman, Matthew
Kolodkin-Gal, Ilana
Ankri, Serge
Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title_full Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title_fullStr Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title_full_unstemmed Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title_short Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
title_sort digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562373/
https://www.ncbi.nlm.nih.gov/pubmed/37813896
http://dx.doi.org/10.1038/s41522-023-00444-x
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