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Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells

Proton therapy is of great interest to pediatric cancer patients because of its optimal depth dose distribution. In view of healthy tissue damage and the increased risk of secondary cancers, we investigated DNA damage induction and repair of radiosensitive hematopoietic stem and progenitor cells (HS...

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Autores principales: Sioen, Simon, Vanhove, Oniecha, Vanderstraeten, Barbara, De Wagter, Carlos, Engelbrecht, Monique, Vandevoorde, Charlot, De Kock, Evan, Van Goethem, Marc-Jan, Vral, Anne, Baeyens, Ans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562436/
https://www.ncbi.nlm.nih.gov/pubmed/37813904
http://dx.doi.org/10.1038/s41598-023-42362-0
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author Sioen, Simon
Vanhove, Oniecha
Vanderstraeten, Barbara
De Wagter, Carlos
Engelbrecht, Monique
Vandevoorde, Charlot
De Kock, Evan
Van Goethem, Marc-Jan
Vral, Anne
Baeyens, Ans
author_facet Sioen, Simon
Vanhove, Oniecha
Vanderstraeten, Barbara
De Wagter, Carlos
Engelbrecht, Monique
Vandevoorde, Charlot
De Kock, Evan
Van Goethem, Marc-Jan
Vral, Anne
Baeyens, Ans
author_sort Sioen, Simon
collection PubMed
description Proton therapy is of great interest to pediatric cancer patients because of its optimal depth dose distribution. In view of healthy tissue damage and the increased risk of secondary cancers, we investigated DNA damage induction and repair of radiosensitive hematopoietic stem and progenitor cells (HSPCs) exposed to therapeutic proton and photon irradiation due to their role in radiation-induced leukemia. Human CD34(+) HSPCs were exposed to 6 MV X-rays, mid- and distal spread-out Bragg peak (SOBP) protons at doses ranging from 0.5 to 2 Gy. Persistent chromosomal damage was assessed with the micronucleus assay, while DNA damage induction and repair were analyzed with the γ-H2AX foci assay. No differences were found in induction and disappearance of γ-H2AX foci between 6 MV X-rays, mid- and distal SOBP protons at 1 Gy. A significantly higher number of micronuclei was found for distal SOBP protons compared to 6 MV X-rays and mid- SOBP protons at 0.5 and 1 Gy, while no significant differences in micronuclei were found at 2 Gy. In HSPCs, mid-SOBP protons are as damaging as conventional X-rays. Distal SOBP protons showed a higher number of micronuclei in HSPCs depending on the radiation dose, indicating possible changes of the in vivo biological response.
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spelling pubmed-105624362023-10-11 Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells Sioen, Simon Vanhove, Oniecha Vanderstraeten, Barbara De Wagter, Carlos Engelbrecht, Monique Vandevoorde, Charlot De Kock, Evan Van Goethem, Marc-Jan Vral, Anne Baeyens, Ans Sci Rep Article Proton therapy is of great interest to pediatric cancer patients because of its optimal depth dose distribution. In view of healthy tissue damage and the increased risk of secondary cancers, we investigated DNA damage induction and repair of radiosensitive hematopoietic stem and progenitor cells (HSPCs) exposed to therapeutic proton and photon irradiation due to their role in radiation-induced leukemia. Human CD34(+) HSPCs were exposed to 6 MV X-rays, mid- and distal spread-out Bragg peak (SOBP) protons at doses ranging from 0.5 to 2 Gy. Persistent chromosomal damage was assessed with the micronucleus assay, while DNA damage induction and repair were analyzed with the γ-H2AX foci assay. No differences were found in induction and disappearance of γ-H2AX foci between 6 MV X-rays, mid- and distal SOBP protons at 1 Gy. A significantly higher number of micronuclei was found for distal SOBP protons compared to 6 MV X-rays and mid- SOBP protons at 0.5 and 1 Gy, while no significant differences in micronuclei were found at 2 Gy. In HSPCs, mid-SOBP protons are as damaging as conventional X-rays. Distal SOBP protons showed a higher number of micronuclei in HSPCs depending on the radiation dose, indicating possible changes of the in vivo biological response. Nature Publishing Group UK 2023-10-09 /pmc/articles/PMC10562436/ /pubmed/37813904 http://dx.doi.org/10.1038/s41598-023-42362-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sioen, Simon
Vanhove, Oniecha
Vanderstraeten, Barbara
De Wagter, Carlos
Engelbrecht, Monique
Vandevoorde, Charlot
De Kock, Evan
Van Goethem, Marc-Jan
Vral, Anne
Baeyens, Ans
Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title_full Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title_fullStr Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title_full_unstemmed Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title_short Impact of proton therapy on the DNA damage induction and repair in hematopoietic stem and progenitor cells
title_sort impact of proton therapy on the dna damage induction and repair in hematopoietic stem and progenitor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562436/
https://www.ncbi.nlm.nih.gov/pubmed/37813904
http://dx.doi.org/10.1038/s41598-023-42362-0
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