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Reversible photoregulation of cell-cell adhesions with opto-E-cadherin

E-cadherin-based cell-cell adhesions are dynamically and locally regulated in many essential processes, including embryogenesis, wound healing and tissue organization, with dysregulation manifesting as tumorigenesis and metastasis. However, the lack of tools that would provide control of the high sp...

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Autores principales: Nzigou Mombo, Brice, Bijonowski, Brent M., Raab, Christopher A., Niland, Stephan, Brockhaus, Katrin, Müller, Marc, Eble, Johannes A., Wegner, Seraphine V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562482/
https://www.ncbi.nlm.nih.gov/pubmed/37813868
http://dx.doi.org/10.1038/s41467-023-41932-0
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author Nzigou Mombo, Brice
Bijonowski, Brent M.
Raab, Christopher A.
Niland, Stephan
Brockhaus, Katrin
Müller, Marc
Eble, Johannes A.
Wegner, Seraphine V.
author_facet Nzigou Mombo, Brice
Bijonowski, Brent M.
Raab, Christopher A.
Niland, Stephan
Brockhaus, Katrin
Müller, Marc
Eble, Johannes A.
Wegner, Seraphine V.
author_sort Nzigou Mombo, Brice
collection PubMed
description E-cadherin-based cell-cell adhesions are dynamically and locally regulated in many essential processes, including embryogenesis, wound healing and tissue organization, with dysregulation manifesting as tumorigenesis and metastasis. However, the lack of tools that would provide control of the high spatiotemporal precision observed with E-cadherin adhesions hampers investigation of the underlying mechanisms. Here, we present an optogenetic tool, opto-E-cadherin, that allows reversible control of E-cadherin-mediated cell-cell adhesions with blue light. With opto-E-cadherin, functionally essential calcium binding is photoregulated such that cells expressing opto-E-cadherin at their surface adhere to each other in the dark but not upon illumination. Consequently, opto-E-cadherin provides remote control over multicellular aggregation, E-cadherin-associated intracellular signalling and F-actin organization in 2D and 3D cell cultures. Opto-E-cadherin also allows switching of multicellular behaviour between single and collective cell migration, as well as of cell invasiveness in vitro and in vivo. Overall, opto-E-cadherin is a powerful optogenetic tool capable of controlling cell-cell adhesions at the molecular, cellular and behavioural level that opens up perspectives for the study of dynamics and spatiotemporal control of E-cadherin in biological processes.
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spelling pubmed-105624822023-10-11 Reversible photoregulation of cell-cell adhesions with opto-E-cadherin Nzigou Mombo, Brice Bijonowski, Brent M. Raab, Christopher A. Niland, Stephan Brockhaus, Katrin Müller, Marc Eble, Johannes A. Wegner, Seraphine V. Nat Commun Article E-cadherin-based cell-cell adhesions are dynamically and locally regulated in many essential processes, including embryogenesis, wound healing and tissue organization, with dysregulation manifesting as tumorigenesis and metastasis. However, the lack of tools that would provide control of the high spatiotemporal precision observed with E-cadherin adhesions hampers investigation of the underlying mechanisms. Here, we present an optogenetic tool, opto-E-cadherin, that allows reversible control of E-cadherin-mediated cell-cell adhesions with blue light. With opto-E-cadherin, functionally essential calcium binding is photoregulated such that cells expressing opto-E-cadherin at their surface adhere to each other in the dark but not upon illumination. Consequently, opto-E-cadherin provides remote control over multicellular aggregation, E-cadherin-associated intracellular signalling and F-actin organization in 2D and 3D cell cultures. Opto-E-cadherin also allows switching of multicellular behaviour between single and collective cell migration, as well as of cell invasiveness in vitro and in vivo. Overall, opto-E-cadherin is a powerful optogenetic tool capable of controlling cell-cell adhesions at the molecular, cellular and behavioural level that opens up perspectives for the study of dynamics and spatiotemporal control of E-cadherin in biological processes. Nature Publishing Group UK 2023-10-09 /pmc/articles/PMC10562482/ /pubmed/37813868 http://dx.doi.org/10.1038/s41467-023-41932-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nzigou Mombo, Brice
Bijonowski, Brent M.
Raab, Christopher A.
Niland, Stephan
Brockhaus, Katrin
Müller, Marc
Eble, Johannes A.
Wegner, Seraphine V.
Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title_full Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title_fullStr Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title_full_unstemmed Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title_short Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
title_sort reversible photoregulation of cell-cell adhesions with opto-e-cadherin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562482/
https://www.ncbi.nlm.nih.gov/pubmed/37813868
http://dx.doi.org/10.1038/s41467-023-41932-0
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