Evaluating the resolution of conventional optical microscopes through point spread function measurement

In the imaging process of conventional optical microscopy, the primary factor hindering microscope resolution is the energy diffusion of incident light, most directly described by the point spread function (PSF). Therefore, accurate calculation and measurement of PSF are essential for evaluating and enhancing imaging resolution. Currently, there are various methods to obtain PSFs, each with different advantages and disadvantages suitable for different scenarios. To provide a comprehensive analysis of PSF-obtaining methods, this study classifies them into four categories based on different acquisition principles and analyzes their advantages and disadvantages, starting from the propagation property of light in optical physics. Finally, two PSF-obtaining methods are proposed based on mathematical modeling and deep learning, demonstrating their effectiveness through experimental results. This study compares and analyzes these results, highlighting the practical applications of image deblurring.