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Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P

Naive T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 recep...

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Autores principales: Garcia-Seyda, Nicolas, Song, Solene, Seveau de Noray, Valentine, David-Broglio, Luc, Matti, Christoph, Artinger, Marc, Dupuy, Florian, Biarnes-Pelicot, Martine, Valignat, Marie-Pierre, Legler, Daniel F., Bajénoff, Marc, Theodoly, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562802/
https://www.ncbi.nlm.nih.gov/pubmed/37822497
http://dx.doi.org/10.1016/j.isci.2023.107695
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author Garcia-Seyda, Nicolas
Song, Solene
Seveau de Noray, Valentine
David-Broglio, Luc
Matti, Christoph
Artinger, Marc
Dupuy, Florian
Biarnes-Pelicot, Martine
Valignat, Marie-Pierre
Legler, Daniel F.
Bajénoff, Marc
Theodoly, Olivier
author_facet Garcia-Seyda, Nicolas
Song, Solene
Seveau de Noray, Valentine
David-Broglio, Luc
Matti, Christoph
Artinger, Marc
Dupuy, Florian
Biarnes-Pelicot, Martine
Valignat, Marie-Pierre
Legler, Daniel F.
Bajénoff, Marc
Theodoly, Olivier
author_sort Garcia-Seyda, Nicolas
collection PubMed
description Naive T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naive T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naive T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our in vitro data thus suggest that naive T lymphocyte chemotax long distances to CCL21 but not toward a source of bioactive S1P.
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spelling pubmed-105628022023-10-11 Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P Garcia-Seyda, Nicolas Song, Solene Seveau de Noray, Valentine David-Broglio, Luc Matti, Christoph Artinger, Marc Dupuy, Florian Biarnes-Pelicot, Martine Valignat, Marie-Pierre Legler, Daniel F. Bajénoff, Marc Theodoly, Olivier iScience Article Naive T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naive T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naive T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our in vitro data thus suggest that naive T lymphocyte chemotax long distances to CCL21 but not toward a source of bioactive S1P. Elsevier 2023-08-19 /pmc/articles/PMC10562802/ /pubmed/37822497 http://dx.doi.org/10.1016/j.isci.2023.107695 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia-Seyda, Nicolas
Song, Solene
Seveau de Noray, Valentine
David-Broglio, Luc
Matti, Christoph
Artinger, Marc
Dupuy, Florian
Biarnes-Pelicot, Martine
Valignat, Marie-Pierre
Legler, Daniel F.
Bajénoff, Marc
Theodoly, Olivier
Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title_full Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title_fullStr Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title_full_unstemmed Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title_short Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P
title_sort naive t lymphocytes chemotax long distance to ccl21 but not to a source of bioactive s1p
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562802/
https://www.ncbi.nlm.nih.gov/pubmed/37822497
http://dx.doi.org/10.1016/j.isci.2023.107695
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