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CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa

Centrosomes play an important role in the microtubule organization of a cell. The sperm's specialized centrosome consists of the canonical barrel-shaped proximal centriole, the funnel-shaped distal centriole, and the pericentriolar material known as striated columns (or segmented columns). Here...

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Autores principales: Turner, Katerina A., Caswell, Drew L., McGrady, Bryce M., Pietras-Allen, Andrea, Sedlak, Jessica, Nathan, Cassandra, Parasuraman, Surya, McGann, Aidan P., Fazili, Farzeen M., Bell, Jonathan R., El Smail, Khalil N., Pillai, Sangeetha B., Parry, Kaelan R., Richardson, Kyle P., Ruble, Kelsie, Jaiswal, Ankit, Shah, Tariq A., Sindhwani, Puneet, Avidor-Reiss, Tomer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562935/
https://www.ncbi.nlm.nih.gov/pubmed/37822686
http://dx.doi.org/10.17912/micropub.biology.000951
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author Turner, Katerina A.
Caswell, Drew L.
McGrady, Bryce M.
Pietras-Allen, Andrea
Sedlak, Jessica
Nathan, Cassandra
Parasuraman, Surya
McGann, Aidan P.
Fazili, Farzeen M.
Bell, Jonathan R.
El Smail, Khalil N.
Pillai, Sangeetha B.
Parry, Kaelan R.
Richardson, Kyle P.
Ruble, Kelsie
Jaiswal, Ankit
Shah, Tariq A.
Sindhwani, Puneet
Avidor-Reiss, Tomer
author_facet Turner, Katerina A.
Caswell, Drew L.
McGrady, Bryce M.
Pietras-Allen, Andrea
Sedlak, Jessica
Nathan, Cassandra
Parasuraman, Surya
McGann, Aidan P.
Fazili, Farzeen M.
Bell, Jonathan R.
El Smail, Khalil N.
Pillai, Sangeetha B.
Parry, Kaelan R.
Richardson, Kyle P.
Ruble, Kelsie
Jaiswal, Ankit
Shah, Tariq A.
Sindhwani, Puneet
Avidor-Reiss, Tomer
author_sort Turner, Katerina A.
collection PubMed
description Centrosomes play an important role in the microtubule organization of a cell. The sperm's specialized centrosome consists of the canonical barrel-shaped proximal centriole, the funnel-shaped distal centriole, and the pericentriolar material known as striated columns (or segmented columns). Here, we examined the localization of the centriole proteins CEP135 and CP110 in cattle and human spermatozoa. In canonical centrioles, CP110 is a centriole tip protein that controls cilia formation, while CEP135 is a structural protein essential for constructing the centriole. In contrast, we found antibodies recognizing CEP135 and CP110 label near the proximal and distal centrioles at the expected location of the striated columns and capitulum in cattle and humans in an antibody and species-specific way. These findings provide a pathway to understanding the unique functions of spermatozoan centrosome.
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spelling pubmed-105629352023-10-11 CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa Turner, Katerina A. Caswell, Drew L. McGrady, Bryce M. Pietras-Allen, Andrea Sedlak, Jessica Nathan, Cassandra Parasuraman, Surya McGann, Aidan P. Fazili, Farzeen M. Bell, Jonathan R. El Smail, Khalil N. Pillai, Sangeetha B. Parry, Kaelan R. Richardson, Kyle P. Ruble, Kelsie Jaiswal, Ankit Shah, Tariq A. Sindhwani, Puneet Avidor-Reiss, Tomer MicroPubl Biol New Finding Centrosomes play an important role in the microtubule organization of a cell. The sperm's specialized centrosome consists of the canonical barrel-shaped proximal centriole, the funnel-shaped distal centriole, and the pericentriolar material known as striated columns (or segmented columns). Here, we examined the localization of the centriole proteins CEP135 and CP110 in cattle and human spermatozoa. In canonical centrioles, CP110 is a centriole tip protein that controls cilia formation, while CEP135 is a structural protein essential for constructing the centriole. In contrast, we found antibodies recognizing CEP135 and CP110 label near the proximal and distal centrioles at the expected location of the striated columns and capitulum in cattle and humans in an antibody and species-specific way. These findings provide a pathway to understanding the unique functions of spermatozoan centrosome. Caltech Library 2023-09-25 /pmc/articles/PMC10562935/ /pubmed/37822686 http://dx.doi.org/10.17912/micropub.biology.000951 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Turner, Katerina A.
Caswell, Drew L.
McGrady, Bryce M.
Pietras-Allen, Andrea
Sedlak, Jessica
Nathan, Cassandra
Parasuraman, Surya
McGann, Aidan P.
Fazili, Farzeen M.
Bell, Jonathan R.
El Smail, Khalil N.
Pillai, Sangeetha B.
Parry, Kaelan R.
Richardson, Kyle P.
Ruble, Kelsie
Jaiswal, Ankit
Shah, Tariq A.
Sindhwani, Puneet
Avidor-Reiss, Tomer
CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title_full CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title_fullStr CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title_full_unstemmed CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title_short CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa
title_sort cp110 and cep135 localize near the proximal and distal centrioles of cattle and human spermatozoa
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562935/
https://www.ncbi.nlm.nih.gov/pubmed/37822686
http://dx.doi.org/10.17912/micropub.biology.000951
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