wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments

MOTIVATION: The automated data processing provided by the TSA-CRAFT tool enables now to reach high throughput speed analysis of thermal shift assays. While the software is powerful and freely available, it still requires installation process and command line efforts that could be discouraging. RESUL...

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Detalles Bibliográficos
Autores principales: Reys, Victor, Kowalewski, Julien, Gelin, Muriel, Lionne, Corinne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Application Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562953/
https://www.ncbi.nlm.nih.gov/pubmed/37822724
http://dx.doi.org/10.1093/bioadv/vbad136
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author Reys, Victor
Kowalewski, Julien
Gelin, Muriel
Lionne, Corinne
author_facet Reys, Victor
Kowalewski, Julien
Gelin, Muriel
Lionne, Corinne
author_sort Reys, Victor
collection PubMed
description MOTIVATION: The automated data processing provided by the TSA-CRAFT tool enables now to reach high throughput speed analysis of thermal shift assays. While the software is powerful and freely available, it still requires installation process and command line efforts that could be discouraging. RESULTS: To simplify the procedure, we decided to make it available and easy to use by implementing it with a graphical interface via a web server, enabling a cross-platform usage from any web browsers. We developed a web server embedded version of the TSA-CRAFT tool, enabling a user-friendly graphical interface for formatting and submission of the input file and visualization of the selected thermal denaturation profiles. We describe a typical case study of buffer condition optimization of the biologically relevant APH(3')-IIb bacterial protein in a 96 deep-well thermal shift analysis screening. AVAILABILITY AND IMPLEMENTATION: wTSA-CRAFT is freely accessible for noncommercial usage at https://bioserv.cbs.cnrs.fr/TSA_CRAFT.
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spelling pubmed-105629532023-10-11 wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments Reys, Victor Kowalewski, Julien Gelin, Muriel Lionne, Corinne Bioinform Adv Application Note MOTIVATION: The automated data processing provided by the TSA-CRAFT tool enables now to reach high throughput speed analysis of thermal shift assays. While the software is powerful and freely available, it still requires installation process and command line efforts that could be discouraging. RESULTS: To simplify the procedure, we decided to make it available and easy to use by implementing it with a graphical interface via a web server, enabling a cross-platform usage from any web browsers. We developed a web server embedded version of the TSA-CRAFT tool, enabling a user-friendly graphical interface for formatting and submission of the input file and visualization of the selected thermal denaturation profiles. We describe a typical case study of buffer condition optimization of the biologically relevant APH(3')-IIb bacterial protein in a 96 deep-well thermal shift analysis screening. AVAILABILITY AND IMPLEMENTATION: wTSA-CRAFT is freely accessible for noncommercial usage at https://bioserv.cbs.cnrs.fr/TSA_CRAFT. Oxford University Press 2023-09-29 /pmc/articles/PMC10562953/ /pubmed/37822724 http://dx.doi.org/10.1093/bioadv/vbad136 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Application Note
Reys, Victor
Kowalewski, Julien
Gelin, Muriel
Lionne, Corinne
wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title_full wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title_fullStr wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title_full_unstemmed wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title_short wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
title_sort wtsa-craft: an open-access web server for rapid analysis of thermal shift assay experiments
topic Application Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562953/
https://www.ncbi.nlm.nih.gov/pubmed/37822724
http://dx.doi.org/10.1093/bioadv/vbad136
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