Cargando…
Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification–Mass Spectrometry
[Image: see text] We present an effective, fast, and user-friendly method to reduce codigestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short preincubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of ly...
Autores principales: | Hollenstein, David M., Maurer-Granofszky, Margarita, Reiter, Wolfgang, Anrather, Dorothea, Gossenreiter, Thomas, Babic, Riccardo, Hartl, Natascha, Kraft, Claudine, Hartl, Markus |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563155/ https://www.ncbi.nlm.nih.gov/pubmed/37712406 http://dx.doi.org/10.1021/acs.jproteome.3c00424 |
Ejemplares similares
-
In Search
of a Universal Method: A Comparative Survey
of Bottom-Up Proteomics Sample Preparation Methods
por: Varnavides, Gina, et al.
Publicado: (2022) -
Production and purification of endogenously modified tRNA-derived small RNAs
por: Drino, Aleksej, et al.
Publicado: (2020) -
Identification of sitagliptin binding proteins by affinity purification mass spectrometry: Sitagliptin binding proteins identified by affinity purification mass spectrometry
por: Wang, Xue-Ning, et al.
Publicado: (2022) -
Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production
por: Talha, Zahir, et al.
Publicado: (2016) -
SAINT: Probabilistic Scoring of Affinity Purification - Mass Spectrometry Data
por: Choi, Hyungwon, et al.
Publicado: (2010)