Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus

[Image: see text] Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extr...

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Autores principales: Chocholova, Eva, Roudnicky, Pavel, Potesil, David, Fialova, Dana, Krystofova, Karolina, Drozdova, Eva, Zdrahal, Zbynek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563166/
https://www.ncbi.nlm.nih.gov/pubmed/37699853
http://dx.doi.org/10.1021/acs.jproteome.3c00370
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author Chocholova, Eva
Roudnicky, Pavel
Potesil, David
Fialova, Dana
Krystofova, Karolina
Drozdova, Eva
Zdrahal, Zbynek
author_facet Chocholova, Eva
Roudnicky, Pavel
Potesil, David
Fialova, Dana
Krystofova, Karolina
Drozdova, Eva
Zdrahal, Zbynek
author_sort Chocholova, Eva
collection PubMed
description [Image: see text] Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
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spelling pubmed-105631662023-10-11 Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus Chocholova, Eva Roudnicky, Pavel Potesil, David Fialova, Dana Krystofova, Karolina Drozdova, Eva Zdrahal, Zbynek J Proteome Res [Image: see text] Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus. American Chemical Society 2023-09-12 /pmc/articles/PMC10563166/ /pubmed/37699853 http://dx.doi.org/10.1021/acs.jproteome.3c00370 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Chocholova, Eva
Roudnicky, Pavel
Potesil, David
Fialova, Dana
Krystofova, Karolina
Drozdova, Eva
Zdrahal, Zbynek
Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title_full Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title_fullStr Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title_full_unstemmed Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title_short Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
title_sort extraction protocol for parallel analysis of proteins and dna from ancient teeth and dental calculus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10563166/
https://www.ncbi.nlm.nih.gov/pubmed/37699853
http://dx.doi.org/10.1021/acs.jproteome.3c00370
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