Cargando…

Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes

Inflammation of chondrocytes plays a critical role in the occurrence and development of osteoarthritis (OA). Recent evidence indicated exosomes derived from mesenchymal stem cells (MSCs-Exos) exhibit excellent anti-inflammatory ability in many troublesome inflammatory diseases including OA. In the p...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Shichao, Jiang, Wenyue, Lv, Shuang, Sun, Zhicheng, Si, Lihui, Hu, Jinxin, Yang, Yang, Qiu, Dingbang, Liu, Xiaobin, Zhu, Siying, Yang, Lunhao, Qi, Ling, Chi, Guangfan, Wang, Guiqing, Li, Pengdong, Liao, Baojian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10564422/
https://www.ncbi.nlm.nih.gov/pubmed/37724890
http://dx.doi.org/10.18632/aging.205034
_version_ 1785118494981881856
author Wang, Shichao
Jiang, Wenyue
Lv, Shuang
Sun, Zhicheng
Si, Lihui
Hu, Jinxin
Yang, Yang
Qiu, Dingbang
Liu, Xiaobin
Zhu, Siying
Yang, Lunhao
Qi, Ling
Chi, Guangfan
Wang, Guiqing
Li, Pengdong
Liao, Baojian
author_facet Wang, Shichao
Jiang, Wenyue
Lv, Shuang
Sun, Zhicheng
Si, Lihui
Hu, Jinxin
Yang, Yang
Qiu, Dingbang
Liu, Xiaobin
Zhu, Siying
Yang, Lunhao
Qi, Ling
Chi, Guangfan
Wang, Guiqing
Li, Pengdong
Liao, Baojian
author_sort Wang, Shichao
collection PubMed
description Inflammation of chondrocytes plays a critical role in the occurrence and development of osteoarthritis (OA). Recent evidence indicated exosomes derived from mesenchymal stem cells (MSCs-Exos) exhibit excellent anti-inflammatory ability in many troublesome inflammatory diseases including OA. In the present study, we aimed to explore the role of human umbilical cord-derived MSCs-Exos (hUC-MSCs-Exos) in treating the inflammation of chondrocytes and its related mechanisms. Ultracentrifugation was applied to isolate hUC-MSCs-Exos from the culture supernatant of hUC-MSCs. Two OA-like in vitro inflammation models of human articular chondrocytes induced with interleukin 1β (IL-1β) and co-incubation with macrophage utilizing transwell cell culture inserts were both used to evaluate the anti-inflammatory effects of hUC-MSCs-Exos. The mRNA sequencing of chondrocytes after treatment and microRNA (miRNA) sequencing of hUC-MSCs-Exos were detected and analyzed for possible mechanism analysis. The results of the study confirmed that hUC-MSCs-Exos had a reversed effect of IL-1β on chondrocytes in the expression of collagen type II alpha 1 (COL2A1) and matrix metalloproteinase 13 (MMP13). The addition of hUC-MSCs-Exos to M1 macrophages in the upper chamber showed down-regulation of IL-1β and tumor necrosis factor α (TNF-α), up-regulation of IL-10 and arginase1 (ARG1), and reversed the gene and protein expression of COL2A1 and MMP13 of the chondrocytes seeded in the lower chamber. The results of this study confirmed the anti-inflammatory effects of hUC-MSCs-Exos in the human articular chondrocytes inflammation model. hUC-MSCs-Exos may be used as a potential cell-free treatment strategy for chondrocyte inflammation in OA.
format Online
Article
Text
id pubmed-10564422
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-105644222023-10-11 Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes Wang, Shichao Jiang, Wenyue Lv, Shuang Sun, Zhicheng Si, Lihui Hu, Jinxin Yang, Yang Qiu, Dingbang Liu, Xiaobin Zhu, Siying Yang, Lunhao Qi, Ling Chi, Guangfan Wang, Guiqing Li, Pengdong Liao, Baojian Aging (Albany NY) Research Paper Inflammation of chondrocytes plays a critical role in the occurrence and development of osteoarthritis (OA). Recent evidence indicated exosomes derived from mesenchymal stem cells (MSCs-Exos) exhibit excellent anti-inflammatory ability in many troublesome inflammatory diseases including OA. In the present study, we aimed to explore the role of human umbilical cord-derived MSCs-Exos (hUC-MSCs-Exos) in treating the inflammation of chondrocytes and its related mechanisms. Ultracentrifugation was applied to isolate hUC-MSCs-Exos from the culture supernatant of hUC-MSCs. Two OA-like in vitro inflammation models of human articular chondrocytes induced with interleukin 1β (IL-1β) and co-incubation with macrophage utilizing transwell cell culture inserts were both used to evaluate the anti-inflammatory effects of hUC-MSCs-Exos. The mRNA sequencing of chondrocytes after treatment and microRNA (miRNA) sequencing of hUC-MSCs-Exos were detected and analyzed for possible mechanism analysis. The results of the study confirmed that hUC-MSCs-Exos had a reversed effect of IL-1β on chondrocytes in the expression of collagen type II alpha 1 (COL2A1) and matrix metalloproteinase 13 (MMP13). The addition of hUC-MSCs-Exos to M1 macrophages in the upper chamber showed down-regulation of IL-1β and tumor necrosis factor α (TNF-α), up-regulation of IL-10 and arginase1 (ARG1), and reversed the gene and protein expression of COL2A1 and MMP13 of the chondrocytes seeded in the lower chamber. The results of this study confirmed the anti-inflammatory effects of hUC-MSCs-Exos in the human articular chondrocytes inflammation model. hUC-MSCs-Exos may be used as a potential cell-free treatment strategy for chondrocyte inflammation in OA. Impact Journals 2023-09-18 /pmc/articles/PMC10564422/ /pubmed/37724890 http://dx.doi.org/10.18632/aging.205034 Text en Copyright: © 2023 Wang et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Shichao
Jiang, Wenyue
Lv, Shuang
Sun, Zhicheng
Si, Lihui
Hu, Jinxin
Yang, Yang
Qiu, Dingbang
Liu, Xiaobin
Zhu, Siying
Yang, Lunhao
Qi, Ling
Chi, Guangfan
Wang, Guiqing
Li, Pengdong
Liao, Baojian
Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title_full Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title_fullStr Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title_full_unstemmed Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title_short Human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
title_sort human umbilical cord mesenchymal stem cells-derived exosomes exert anti-inflammatory effects on osteoarthritis chondrocytes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10564422/
https://www.ncbi.nlm.nih.gov/pubmed/37724890
http://dx.doi.org/10.18632/aging.205034
work_keys_str_mv AT wangshichao humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT jiangwenyue humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT lvshuang humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT sunzhicheng humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT silihui humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT hujinxin humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT yangyang humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT qiudingbang humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT liuxiaobin humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT zhusiying humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT yanglunhao humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT qiling humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT chiguangfan humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT wangguiqing humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT lipengdong humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes
AT liaobaojian humanumbilicalcordmesenchymalstemcellsderivedexosomesexertantiinflammatoryeffectsonosteoarthritischondrocytes