Light sheet fluorescence microscopy of cleared human eyes

We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, includin...

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Detalles Bibliográficos
Autores principales: Darche, Marie, Borella, Ysé, Verschueren, Anna, Gantar, Ivana, Pagès, Stéphane, Batti, Laura, Paques, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10564773/
https://www.ncbi.nlm.nih.gov/pubmed/37816868
http://dx.doi.org/10.1038/s42003-023-05401-0

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