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Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India
BACKGROUND: It has been amply described that levels of IgM antibodies against Mycobacterium leprae (M. leprae) phenolic glycolipid I (PGL-I) correlate strongly with the bacterial load in an infected individual. These findings have generated the concept of using seropositivity for antibodies against...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565223/ https://www.ncbi.nlm.nih.gov/pubmed/37828950 http://dx.doi.org/10.3389/fmed.2023.1260375 |
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author | Pierneef, Louise Malaviya, Paritosh van Hooij, Anouk Sundar, Shyam Singh, Abhishek Kumar Kumar, Rajiv de Jong, Danielle Meuldijk, Maaike Kumar, Awnish Zhou, Zijie Cloots, Kristien Corstjens, Paul Hasker, Epco Geluk, Annemieke |
author_facet | Pierneef, Louise Malaviya, Paritosh van Hooij, Anouk Sundar, Shyam Singh, Abhishek Kumar Kumar, Rajiv de Jong, Danielle Meuldijk, Maaike Kumar, Awnish Zhou, Zijie Cloots, Kristien Corstjens, Paul Hasker, Epco Geluk, Annemieke |
author_sort | Pierneef, Louise |
collection | PubMed |
description | BACKGROUND: It has been amply described that levels of IgM antibodies against Mycobacterium leprae (M. leprae) phenolic glycolipid I (PGL-I) correlate strongly with the bacterial load in an infected individual. These findings have generated the concept of using seropositivity for antibodies against M. leprae PGL-I as an indicator of the proportion of the population that has been infected. Although anti-PGL-I IgM levels provide information on whether an individual has ever been infected, their presence cannot discriminate between recent and past infections. Since infection in (young) children by definition indicates recent transmission, we piloted the feasibility of assessment of anti-PGL-I IgM seroprevalence among children in a leprosy endemic area in India as a proxy for recent M. leprae transmission. MATERIAL AND METHODS: A serosurvey for anti-PGL-I IgM antibodies among children in highly leprosy endemic villages in Bihar, India, was performed, applying the quantitative anti-PGL-I UCP-LFA cassette combined with low-invasive, small-volume fingerstick blood (FSB). RESULTS: Local staff obtained FSB of 1,857 children (age 3–11 years) living in 12 leprosy endemic villages in Bihar; of these, 215 children (11.58%) were seropositive for anti-PGL-I IgM. CONCLUSION: The anti-PGL-I seroprevalence level of 11.58% among children corresponds with the seroprevalence levels described in studies in other leprosy endemic areas over the past decades where no prophylactic interventions have taken place. The anti-PGL-I UCP-LFA was found to be a low-complexity tool that could be practically combined with serosurveys and was well-accepted by both healthcare staff and the population. On route to leprosy elimination, quantitative anti-PGL-I serology in young children holds promise as a strategy to monitor recent M. leprae transmission in an area. |
format | Online Article Text |
id | pubmed-10565223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105652232023-10-12 Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India Pierneef, Louise Malaviya, Paritosh van Hooij, Anouk Sundar, Shyam Singh, Abhishek Kumar Kumar, Rajiv de Jong, Danielle Meuldijk, Maaike Kumar, Awnish Zhou, Zijie Cloots, Kristien Corstjens, Paul Hasker, Epco Geluk, Annemieke Front Med (Lausanne) Medicine BACKGROUND: It has been amply described that levels of IgM antibodies against Mycobacterium leprae (M. leprae) phenolic glycolipid I (PGL-I) correlate strongly with the bacterial load in an infected individual. These findings have generated the concept of using seropositivity for antibodies against M. leprae PGL-I as an indicator of the proportion of the population that has been infected. Although anti-PGL-I IgM levels provide information on whether an individual has ever been infected, their presence cannot discriminate between recent and past infections. Since infection in (young) children by definition indicates recent transmission, we piloted the feasibility of assessment of anti-PGL-I IgM seroprevalence among children in a leprosy endemic area in India as a proxy for recent M. leprae transmission. MATERIAL AND METHODS: A serosurvey for anti-PGL-I IgM antibodies among children in highly leprosy endemic villages in Bihar, India, was performed, applying the quantitative anti-PGL-I UCP-LFA cassette combined with low-invasive, small-volume fingerstick blood (FSB). RESULTS: Local staff obtained FSB of 1,857 children (age 3–11 years) living in 12 leprosy endemic villages in Bihar; of these, 215 children (11.58%) were seropositive for anti-PGL-I IgM. CONCLUSION: The anti-PGL-I seroprevalence level of 11.58% among children corresponds with the seroprevalence levels described in studies in other leprosy endemic areas over the past decades where no prophylactic interventions have taken place. The anti-PGL-I UCP-LFA was found to be a low-complexity tool that could be practically combined with serosurveys and was well-accepted by both healthcare staff and the population. On route to leprosy elimination, quantitative anti-PGL-I serology in young children holds promise as a strategy to monitor recent M. leprae transmission in an area. Frontiers Media S.A. 2023-09-27 /pmc/articles/PMC10565223/ /pubmed/37828950 http://dx.doi.org/10.3389/fmed.2023.1260375 Text en Copyright © 2023 Pierneef, Malaviya, van Hooij, Sundar, Singh, Kumar, de Jong, Meuldijk, Kumar, Zhou, Cloots, Corstjens, Hasker and Geluk. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Pierneef, Louise Malaviya, Paritosh van Hooij, Anouk Sundar, Shyam Singh, Abhishek Kumar Kumar, Rajiv de Jong, Danielle Meuldijk, Maaike Kumar, Awnish Zhou, Zijie Cloots, Kristien Corstjens, Paul Hasker, Epco Geluk, Annemieke Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title | Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title_full | Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title_fullStr | Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title_full_unstemmed | Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title_short | Field-friendly anti-PGL-I serosurvey in children to monitor Mycobacterium leprae transmission in Bihar, India |
title_sort | field-friendly anti-pgl-i serosurvey in children to monitor mycobacterium leprae transmission in bihar, india |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565223/ https://www.ncbi.nlm.nih.gov/pubmed/37828950 http://dx.doi.org/10.3389/fmed.2023.1260375 |
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