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Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions

INTRODUCTION: Trans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from t...

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Autores principales: Alburquerque, Nuria, Pérez-Caselles, Cristian, Faize, Lydia, Ilardi, Vincenza, Burgos, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565502/
https://www.ncbi.nlm.nih.gov/pubmed/37828929
http://dx.doi.org/10.3389/fpls.2023.1216217
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author Alburquerque, Nuria
Pérez-Caselles, Cristian
Faize, Lydia
Ilardi, Vincenza
Burgos, Lorenzo
author_facet Alburquerque, Nuria
Pérez-Caselles, Cristian
Faize, Lydia
Ilardi, Vincenza
Burgos, Lorenzo
author_sort Alburquerque, Nuria
collection PubMed
description INTRODUCTION: Trans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from transgenic plum rootstocks to wild-type apricot scions grafted onto them. METHODS: To this end, we conducted grafting experiments of wild- type apricots onto plum plants transformed with a construction codifying a hairpin RNA designed to silence the PPV virus and studied if the resistance was transmitted from the rootstock to the scion. RESULTS: Our data support that the RNA-silencing-based PPV resistance can be transmitted from PPV-resistant plum rootstocks to non-transgenic apricot scions and that its efficiency is augmented after successive growth cycles. PPV resistance conferred by the rootstocks was robust, already occurring within the same growing cycle and maintained in successive evaluation cycles. The RNA silencing mechanism reduces the relative accumulation of the virus progressively eliminating the virus from the wild type scions grafted on the transgenic resistant PPV plants. There was a preferential accumulation of the 24nt siRNAs in the scions grafted onto resistant rootstocks that was not found in the scions grafted on the susceptible rootstock. This matched with a significantly lower relative accumulation of hpRNA in the resistant rootstocks compared with the susceptible or the tolerant ones. DISCUSSION: Using transgenic rootstocks should mitigate public concerns about transgenes dispersion and eating transgenic food and allow conferring virus resistance to recalcitrant to transformation cultivars or species.
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spelling pubmed-105655022023-10-12 Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions Alburquerque, Nuria Pérez-Caselles, Cristian Faize, Lydia Ilardi, Vincenza Burgos, Lorenzo Front Plant Sci Plant Science INTRODUCTION: Trans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from transgenic plum rootstocks to wild-type apricot scions grafted onto them. METHODS: To this end, we conducted grafting experiments of wild- type apricots onto plum plants transformed with a construction codifying a hairpin RNA designed to silence the PPV virus and studied if the resistance was transmitted from the rootstock to the scion. RESULTS: Our data support that the RNA-silencing-based PPV resistance can be transmitted from PPV-resistant plum rootstocks to non-transgenic apricot scions and that its efficiency is augmented after successive growth cycles. PPV resistance conferred by the rootstocks was robust, already occurring within the same growing cycle and maintained in successive evaluation cycles. The RNA silencing mechanism reduces the relative accumulation of the virus progressively eliminating the virus from the wild type scions grafted on the transgenic resistant PPV plants. There was a preferential accumulation of the 24nt siRNAs in the scions grafted onto resistant rootstocks that was not found in the scions grafted on the susceptible rootstock. This matched with a significantly lower relative accumulation of hpRNA in the resistant rootstocks compared with the susceptible or the tolerant ones. DISCUSSION: Using transgenic rootstocks should mitigate public concerns about transgenes dispersion and eating transgenic food and allow conferring virus resistance to recalcitrant to transformation cultivars or species. Frontiers Media S.A. 2023-09-27 /pmc/articles/PMC10565502/ /pubmed/37828929 http://dx.doi.org/10.3389/fpls.2023.1216217 Text en Copyright © 2023 Alburquerque, Pérez-Caselles, Faize, Ilardi and Burgos https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Alburquerque, Nuria
Pérez-Caselles, Cristian
Faize, Lydia
Ilardi, Vincenza
Burgos, Lorenzo
Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title_full Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title_fullStr Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title_full_unstemmed Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title_short Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
title_sort trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565502/
https://www.ncbi.nlm.nih.gov/pubmed/37828929
http://dx.doi.org/10.3389/fpls.2023.1216217
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