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Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we pe...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565639/ https://www.ncbi.nlm.nih.gov/pubmed/37522383 http://dx.doi.org/10.15252/emmm.202217240 |
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author | Argyriou, Alexandra Horuluoglu, Begum Galindo‐Feria, Angeles Shunashy Diaz‐Boada, Juan Sebastian Sijbranda, Merel Notarnicola, Antonella Dani, Lara van Vollenhoven, Annika Ramsköld, Daniel Nennesmo, Inger Dastmalchi, Maryam Lundberg, Ingrid E Diaz‐Gallo, Lina‐Marcela Chemin, Karine |
author_facet | Argyriou, Alexandra Horuluoglu, Begum Galindo‐Feria, Angeles Shunashy Diaz‐Boada, Juan Sebastian Sijbranda, Merel Notarnicola, Antonella Dani, Lara van Vollenhoven, Annika Ramsköld, Daniel Nennesmo, Inger Dastmalchi, Maryam Lundberg, Ingrid E Diaz‐Gallo, Lina‐Marcela Chemin, Karine |
author_sort | Argyriou, Alexandra |
collection | PubMed |
description | Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we performed in‐depth single‐cell sequencing on muscle‐infiltrating T cells and peripheral blood memory T cells in six patients with recently diagnosed IIM. We identified tissue resident memory T‐cell (T(RM)) signatures including the expression of HOBIT, XCL1 and CXCR6 in the muscle biopsies of all patients with IIM. Clonally expanded T‐cell clones were mainly found among cytotoxic and T(RM) implying their role in the disease pathogenesis. Finally, identical expanded T‐cell clones persisting at follow‐up in the muscle tissue of two patients suggest their involvement in disease chronicity. Our study reveals a muscle tissue resident memory T‐cell signature in patients with IIM and a transcriptomic map to identify novel therapeutic targets in IIM. |
format | Online Article Text |
id | pubmed-10565639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105656392023-10-12 Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies Argyriou, Alexandra Horuluoglu, Begum Galindo‐Feria, Angeles Shunashy Diaz‐Boada, Juan Sebastian Sijbranda, Merel Notarnicola, Antonella Dani, Lara van Vollenhoven, Annika Ramsköld, Daniel Nennesmo, Inger Dastmalchi, Maryam Lundberg, Ingrid E Diaz‐Gallo, Lina‐Marcela Chemin, Karine EMBO Mol Med Resource Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we performed in‐depth single‐cell sequencing on muscle‐infiltrating T cells and peripheral blood memory T cells in six patients with recently diagnosed IIM. We identified tissue resident memory T‐cell (T(RM)) signatures including the expression of HOBIT, XCL1 and CXCR6 in the muscle biopsies of all patients with IIM. Clonally expanded T‐cell clones were mainly found among cytotoxic and T(RM) implying their role in the disease pathogenesis. Finally, identical expanded T‐cell clones persisting at follow‐up in the muscle tissue of two patients suggest their involvement in disease chronicity. Our study reveals a muscle tissue resident memory T‐cell signature in patients with IIM and a transcriptomic map to identify novel therapeutic targets in IIM. John Wiley and Sons Inc. 2023-07-31 /pmc/articles/PMC10565639/ /pubmed/37522383 http://dx.doi.org/10.15252/emmm.202217240 Text en © 2023 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Resource Argyriou, Alexandra Horuluoglu, Begum Galindo‐Feria, Angeles Shunashy Diaz‐Boada, Juan Sebastian Sijbranda, Merel Notarnicola, Antonella Dani, Lara van Vollenhoven, Annika Ramsköld, Daniel Nennesmo, Inger Dastmalchi, Maryam Lundberg, Ingrid E Diaz‐Gallo, Lina‐Marcela Chemin, Karine Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title | Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title_full | Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title_fullStr | Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title_full_unstemmed | Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title_short | Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies |
title_sort | single‐cell profiling of muscle‐infiltrating t cells in idiopathic inflammatory myopathies |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565639/ https://www.ncbi.nlm.nih.gov/pubmed/37522383 http://dx.doi.org/10.15252/emmm.202217240 |
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