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Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies

Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we pe...

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Autores principales: Argyriou, Alexandra, Horuluoglu, Begum, Galindo‐Feria, Angeles Shunashy, Diaz‐Boada, Juan Sebastian, Sijbranda, Merel, Notarnicola, Antonella, Dani, Lara, van Vollenhoven, Annika, Ramsköld, Daniel, Nennesmo, Inger, Dastmalchi, Maryam, Lundberg, Ingrid E, Diaz‐Gallo, Lina‐Marcela, Chemin, Karine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565639/
https://www.ncbi.nlm.nih.gov/pubmed/37522383
http://dx.doi.org/10.15252/emmm.202217240
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author Argyriou, Alexandra
Horuluoglu, Begum
Galindo‐Feria, Angeles Shunashy
Diaz‐Boada, Juan Sebastian
Sijbranda, Merel
Notarnicola, Antonella
Dani, Lara
van Vollenhoven, Annika
Ramsköld, Daniel
Nennesmo, Inger
Dastmalchi, Maryam
Lundberg, Ingrid E
Diaz‐Gallo, Lina‐Marcela
Chemin, Karine
author_facet Argyriou, Alexandra
Horuluoglu, Begum
Galindo‐Feria, Angeles Shunashy
Diaz‐Boada, Juan Sebastian
Sijbranda, Merel
Notarnicola, Antonella
Dani, Lara
van Vollenhoven, Annika
Ramsköld, Daniel
Nennesmo, Inger
Dastmalchi, Maryam
Lundberg, Ingrid E
Diaz‐Gallo, Lina‐Marcela
Chemin, Karine
author_sort Argyriou, Alexandra
collection PubMed
description Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we performed in‐depth single‐cell sequencing on muscle‐infiltrating T cells and peripheral blood memory T cells in six patients with recently diagnosed IIM. We identified tissue resident memory T‐cell (T(RM)) signatures including the expression of HOBIT, XCL1 and CXCR6 in the muscle biopsies of all patients with IIM. Clonally expanded T‐cell clones were mainly found among cytotoxic and T(RM) implying their role in the disease pathogenesis. Finally, identical expanded T‐cell clones persisting at follow‐up in the muscle tissue of two patients suggest their involvement in disease chronicity. Our study reveals a muscle tissue resident memory T‐cell signature in patients with IIM and a transcriptomic map to identify novel therapeutic targets in IIM.
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spelling pubmed-105656392023-10-12 Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies Argyriou, Alexandra Horuluoglu, Begum Galindo‐Feria, Angeles Shunashy Diaz‐Boada, Juan Sebastian Sijbranda, Merel Notarnicola, Antonella Dani, Lara van Vollenhoven, Annika Ramsköld, Daniel Nennesmo, Inger Dastmalchi, Maryam Lundberg, Ingrid E Diaz‐Gallo, Lina‐Marcela Chemin, Karine EMBO Mol Med Resource Idiopathic inflammatory myopathies (IIM) are rare autoimmune systemic diseases characterized by muscle weakness and the presence of muscle‐infiltrating T cells. IIM represent a clinical challenge due to heterogeneity of symptoms and variability of response to immunosuppressive treatment. Here, we performed in‐depth single‐cell sequencing on muscle‐infiltrating T cells and peripheral blood memory T cells in six patients with recently diagnosed IIM. We identified tissue resident memory T‐cell (T(RM)) signatures including the expression of HOBIT, XCL1 and CXCR6 in the muscle biopsies of all patients with IIM. Clonally expanded T‐cell clones were mainly found among cytotoxic and T(RM) implying their role in the disease pathogenesis. Finally, identical expanded T‐cell clones persisting at follow‐up in the muscle tissue of two patients suggest their involvement in disease chronicity. Our study reveals a muscle tissue resident memory T‐cell signature in patients with IIM and a transcriptomic map to identify novel therapeutic targets in IIM. John Wiley and Sons Inc. 2023-07-31 /pmc/articles/PMC10565639/ /pubmed/37522383 http://dx.doi.org/10.15252/emmm.202217240 Text en © 2023 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Resource
Argyriou, Alexandra
Horuluoglu, Begum
Galindo‐Feria, Angeles Shunashy
Diaz‐Boada, Juan Sebastian
Sijbranda, Merel
Notarnicola, Antonella
Dani, Lara
van Vollenhoven, Annika
Ramsköld, Daniel
Nennesmo, Inger
Dastmalchi, Maryam
Lundberg, Ingrid E
Diaz‐Gallo, Lina‐Marcela
Chemin, Karine
Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title_full Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title_fullStr Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title_full_unstemmed Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title_short Single‐cell profiling of muscle‐infiltrating T cells in idiopathic inflammatory myopathies
title_sort single‐cell profiling of muscle‐infiltrating t cells in idiopathic inflammatory myopathies
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565639/
https://www.ncbi.nlm.nih.gov/pubmed/37522383
http://dx.doi.org/10.15252/emmm.202217240
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