Protocol for selecting single human pluripotent stem cells using a modified micropipetter

Single-cell clonal selection is a critical procedure for generating a homogeneous population of human pluripotent stem cells. Here, we present a protocol that repurposes the STRIPPER Micropipetter, normally used for in vitro fertilization, to pick single stem cells. We describe steps for tool and re...

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Detalles Bibliográficos
Autores principales: Mojica-Perez, Sandra P., Stokes, Kyle, Jaklic, Daniel C., Jahagirdar, Sheetal, Uhler, Michael, Parent, Jack M., Niu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565870/
https://www.ncbi.nlm.nih.gov/pubmed/37804512
http://dx.doi.org/10.1016/j.xpro.2023.102629

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