Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole

Four simple, specific, easy, precise and accurate spectrophotometric methods were developed for the first time to examine ciprofloxacin and metronidazole in combination, without having been separated beforehand by the developed methods. Ciprofloxacin and metronidazole were determined by utilizing ad...

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Autores principales: Sebaiy, Mahmoud M., El-Adl, Sobhy M., Nafea, Alaa, Aljazzar, Samar O., Elkaeed, Eslam B., Mattar, Amr A., Elbaramawi, Samar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10566196/
https://www.ncbi.nlm.nih.gov/pubmed/37817284
http://dx.doi.org/10.1186/s13065-023-01007-z
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author Sebaiy, Mahmoud M.
El-Adl, Sobhy M.
Nafea, Alaa
Aljazzar, Samar O.
Elkaeed, Eslam B.
Mattar, Amr A.
Elbaramawi, Samar S.
author_facet Sebaiy, Mahmoud M.
El-Adl, Sobhy M.
Nafea, Alaa
Aljazzar, Samar O.
Elkaeed, Eslam B.
Mattar, Amr A.
Elbaramawi, Samar S.
author_sort Sebaiy, Mahmoud M.
collection PubMed
description Four simple, specific, easy, precise and accurate spectrophotometric methods were developed for the first time to examine ciprofloxacin and metronidazole in combination, without having been separated beforehand by the developed methods. Ciprofloxacin and metronidazole were determined by utilizing advanced absorbance subtraction (AAS), spectrum subtraction, bivariate and ratio difference methods. Precision, repeatability, robustness, and accuracy were all determined to be within acceptable levels after each of these procedures underwent validation in accordance with ICH recommendations. Each method’s benefits and drawbacks are illustrated, and the proposed and reported methodologies were statistically compared. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13065-023-01007-z.
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spelling pubmed-105661962023-10-12 Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole Sebaiy, Mahmoud M. El-Adl, Sobhy M. Nafea, Alaa Aljazzar, Samar O. Elkaeed, Eslam B. Mattar, Amr A. Elbaramawi, Samar S. BMC Chem Research Four simple, specific, easy, precise and accurate spectrophotometric methods were developed for the first time to examine ciprofloxacin and metronidazole in combination, without having been separated beforehand by the developed methods. Ciprofloxacin and metronidazole were determined by utilizing advanced absorbance subtraction (AAS), spectrum subtraction, bivariate and ratio difference methods. Precision, repeatability, robustness, and accuracy were all determined to be within acceptable levels after each of these procedures underwent validation in accordance with ICH recommendations. Each method’s benefits and drawbacks are illustrated, and the proposed and reported methodologies were statistically compared. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13065-023-01007-z. Springer International Publishing 2023-10-10 /pmc/articles/PMC10566196/ /pubmed/37817284 http://dx.doi.org/10.1186/s13065-023-01007-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sebaiy, Mahmoud M.
El-Adl, Sobhy M.
Nafea, Alaa
Aljazzar, Samar O.
Elkaeed, Eslam B.
Mattar, Amr A.
Elbaramawi, Samar S.
Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title_full Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title_fullStr Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title_full_unstemmed Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title_short Different methods for resolving overlapping UV spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
title_sort different methods for resolving overlapping uv spectra of combination medicinal dose forms of ciprofloxacin and metronidazole
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10566196/
https://www.ncbi.nlm.nih.gov/pubmed/37817284
http://dx.doi.org/10.1186/s13065-023-01007-z
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