High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq

In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further a...

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Detalles Bibliográficos
Autores principales: Liu, Yang, DiStasio, Marcello, Su, Graham, Asashima, Hiromitsu, Enninful, Archibald, Qin, Xiaoyu, Deng, Yanxiang, Nam, Jungmin, Gao, Fu, Bordignon, Pino, Cassano, Marco, Tomayko, Mary, Xu, Mina, Halene, Stephanie, Craft, Joseph E., Hafler, David, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567548/
https://www.ncbi.nlm.nih.gov/pubmed/36823353
http://dx.doi.org/10.1038/s41587-023-01676-0
Descripción
Sumario:In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.

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