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“Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line
The cultivar “Nulichal,” a type of naked waxy barley (Hordeum vulgare L.), was developed by the National Institute of Crop Science, Rural Development Administration, Korea, in 2010. In this study, we investigated the anti-inflammatory and antioxidant properties of the “Nulichal” ethanol extract (NRE...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society of Food Science and Nutrition
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567604/ https://www.ncbi.nlm.nih.gov/pubmed/37842259 http://dx.doi.org/10.3746/pnf.2023.28.3.370 |
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author | Han, Jisu Ham, Ju Ri Lee, Mi Ja Lee, Hyun-Jin Son, Young-Jin Lee, Mi-Kyung |
author_facet | Han, Jisu Ham, Ju Ri Lee, Mi Ja Lee, Hyun-Jin Son, Young-Jin Lee, Mi-Kyung |
author_sort | Han, Jisu |
collection | PubMed |
description | The cultivar “Nulichal,” a type of naked waxy barley (Hordeum vulgare L.), was developed by the National Institute of Crop Science, Rural Development Administration, Korea, in 2010. In this study, we investigated the anti-inflammatory and antioxidant properties of the “Nulichal” ethanol extract (NRE) using various assays. The NRE exhibited a total phenolic content of 7.55±0.30 mg gallic acid equivalent/g and a flavonoid content of 1.74±0.08 mg rutin equivalent/g. Cell viability assays showed no toxicity of NRE on RAW264.7 macrophage cells up to concentrations of 500 μg/mL. The NRE (300 and 500 μg/mL) significantly reduced nitric oxide (NO) production induced by lipopolysaccharides (LPS). It also down-regulated the mRNA expression and protein levels of inducible NO synthase and cyclooxygenase-2 in a dose-dependent manner. Moreover, the NRE treatment significantly decreased the levels of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, and their mRNA expression compared to LPS treatment alone. The NRE demonstrated strong free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals in a dose-dependent manner. The ferric reducing antioxidant power assay also showed increased antioxidant activity with increasing NRE concentrations. These findings suggest that the NRE can be used as a functional food with anti-inflammatory and antioxidant properties. |
format | Online Article Text |
id | pubmed-10567604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Korean Society of Food Science and Nutrition |
record_format | MEDLINE/PubMed |
spelling | pubmed-105676042023-10-13 “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line Han, Jisu Ham, Ju Ri Lee, Mi Ja Lee, Hyun-Jin Son, Young-Jin Lee, Mi-Kyung Prev Nutr Food Sci Note The cultivar “Nulichal,” a type of naked waxy barley (Hordeum vulgare L.), was developed by the National Institute of Crop Science, Rural Development Administration, Korea, in 2010. In this study, we investigated the anti-inflammatory and antioxidant properties of the “Nulichal” ethanol extract (NRE) using various assays. The NRE exhibited a total phenolic content of 7.55±0.30 mg gallic acid equivalent/g and a flavonoid content of 1.74±0.08 mg rutin equivalent/g. Cell viability assays showed no toxicity of NRE on RAW264.7 macrophage cells up to concentrations of 500 μg/mL. The NRE (300 and 500 μg/mL) significantly reduced nitric oxide (NO) production induced by lipopolysaccharides (LPS). It also down-regulated the mRNA expression and protein levels of inducible NO synthase and cyclooxygenase-2 in a dose-dependent manner. Moreover, the NRE treatment significantly decreased the levels of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, and their mRNA expression compared to LPS treatment alone. The NRE demonstrated strong free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals in a dose-dependent manner. The ferric reducing antioxidant power assay also showed increased antioxidant activity with increasing NRE concentrations. These findings suggest that the NRE can be used as a functional food with anti-inflammatory and antioxidant properties. The Korean Society of Food Science and Nutrition 2023-09-30 2023-09-30 /pmc/articles/PMC10567604/ /pubmed/37842259 http://dx.doi.org/10.3746/pnf.2023.28.3.370 Text en Copyright © 2023 by The Korean Society of Food Science and Nutrition. https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Note Han, Jisu Ham, Ju Ri Lee, Mi Ja Lee, Hyun-Jin Son, Young-Jin Lee, Mi-Kyung “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title | “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title_full | “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title_fullStr | “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title_full_unstemmed | “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title_short | “Nulichal” Barley Extract Suppresses Nitric Oxide and Pro-Inflammatory Cytokine Production by Lipopolysaccharides in RAW264.7 Macrophage Cell Line |
title_sort | “nulichal” barley extract suppresses nitric oxide and pro-inflammatory cytokine production by lipopolysaccharides in raw264.7 macrophage cell line |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567604/ https://www.ncbi.nlm.nih.gov/pubmed/37842259 http://dx.doi.org/10.3746/pnf.2023.28.3.370 |
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