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Laser nanostructured gold biosensor for proto-oncogene detection
The advancement of biosensor research has been a primary driving force in the continuing progress of modern medical science. While traditional nanofabrication methods have long been the foundation of biosensor research, recent years have seen a shift in the field of nanofabrication towards laser-bas...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567688/ https://www.ncbi.nlm.nih.gov/pubmed/37821490 http://dx.doi.org/10.1038/s41598-023-44372-4 |
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author | Hughes, Cian Sreenilayam, Sithara Brabazon, Dermot |
author_facet | Hughes, Cian Sreenilayam, Sithara Brabazon, Dermot |
author_sort | Hughes, Cian |
collection | PubMed |
description | The advancement of biosensor research has been a primary driving force in the continuing progress of modern medical science. While traditional nanofabrication methods have long been the foundation of biosensor research, recent years have seen a shift in the field of nanofabrication towards laser-based techniques. Here we report a gold-based biosensor, with a limit of detection (LoD) 3.18 µM, developed using environmentally friendly Laser Ablation Synthesis in Liquid (LASiS) and Confined Atmospheric Pulsed-laser (CAP) deposition techniques for the first time. The sensors were able detect a DNA fragment corresponding to the longest unpaired sequence of the c-Myc gene, indicating their potential for detecting such fragments in the ctDNA signature of various cancers. The LoD of the developed novel biosensor highlights its reliability and sensitivity as an analytical platform. The reproducibility of the sensor was examined via the production and testing of 200 sensors with the same fabrication methodology. This work offers a scalable, and green approach to fabricating viable biosensors capable of detecting clinically relevant oncogenic targets. |
format | Online Article Text |
id | pubmed-10567688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105676882023-10-13 Laser nanostructured gold biosensor for proto-oncogene detection Hughes, Cian Sreenilayam, Sithara Brabazon, Dermot Sci Rep Article The advancement of biosensor research has been a primary driving force in the continuing progress of modern medical science. While traditional nanofabrication methods have long been the foundation of biosensor research, recent years have seen a shift in the field of nanofabrication towards laser-based techniques. Here we report a gold-based biosensor, with a limit of detection (LoD) 3.18 µM, developed using environmentally friendly Laser Ablation Synthesis in Liquid (LASiS) and Confined Atmospheric Pulsed-laser (CAP) deposition techniques for the first time. The sensors were able detect a DNA fragment corresponding to the longest unpaired sequence of the c-Myc gene, indicating their potential for detecting such fragments in the ctDNA signature of various cancers. The LoD of the developed novel biosensor highlights its reliability and sensitivity as an analytical platform. The reproducibility of the sensor was examined via the production and testing of 200 sensors with the same fabrication methodology. This work offers a scalable, and green approach to fabricating viable biosensors capable of detecting clinically relevant oncogenic targets. Nature Publishing Group UK 2023-10-11 /pmc/articles/PMC10567688/ /pubmed/37821490 http://dx.doi.org/10.1038/s41598-023-44372-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hughes, Cian Sreenilayam, Sithara Brabazon, Dermot Laser nanostructured gold biosensor for proto-oncogene detection |
title | Laser nanostructured gold biosensor for proto-oncogene detection |
title_full | Laser nanostructured gold biosensor for proto-oncogene detection |
title_fullStr | Laser nanostructured gold biosensor for proto-oncogene detection |
title_full_unstemmed | Laser nanostructured gold biosensor for proto-oncogene detection |
title_short | Laser nanostructured gold biosensor for proto-oncogene detection |
title_sort | laser nanostructured gold biosensor for proto-oncogene detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567688/ https://www.ncbi.nlm.nih.gov/pubmed/37821490 http://dx.doi.org/10.1038/s41598-023-44372-4 |
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