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Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli

Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each with a single amino acid change in one of 346 essential proteins. 1,269 of these mutants showed temperature‐sensitive growth...

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Detalles Bibliográficos
Autores principales:	Schramm, Thorben, Lubrano, Paul, Pahl, Vanessa, Stadelmann, Amelie, Verhülsdonk, Andreas, Link, Hannes
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2023
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568205/ https://www.ncbi.nlm.nih.gov/pubmed/37642940 http://dx.doi.org/10.15252/msb.202311596

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