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Electroporation-based Easi-CRISPR yields biallelic insertions of EGFP-HiBiT cassette in immortalized chicken oviduct epithelial cells

Laying hens are an excellent experimental oviduct model for studying reproduction biology. Because chicken oviduct epithelial cells (cOECs) have a crucial role in synthesizing and secreting ovalbumin, laying hens have been regarded an ideal bioreactor for producing pharmaceuticals in egg white throu...

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Detalles Bibliográficos
Autores principales: Liu, Lingkang, Wei, Jinyu, Chen, Chen, Liang, Qianxue, Wang, Boyong, Wu, Wende, Li, Gonghe, Zheng, Xibang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568294/
https://www.ncbi.nlm.nih.gov/pubmed/37806084
http://dx.doi.org/10.1016/j.psj.2023.103112