Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma

BACKGROUND: In recent years, hyperthermia has been widely applied as a novel strategy for cancer treatment due to its multiple antitumour effects. In particular, the potential influences of hyperthermia on the tumour immune microenvironment may improve the efficacy of immunotherapies. However, the e...

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Autores principales: Huaqi, Yin, Bingqi, Dong, Yanhui, Zhao, Yongkang, Ma, Shiming, Zhao, Zhenghui, Sun, Zheng, Du, Jiangshan, Peng, Tiejun, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568811/
https://www.ncbi.nlm.nih.gov/pubmed/37828458
http://dx.doi.org/10.1186/s12885-023-11106-8
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author Huaqi, Yin
Bingqi, Dong
Yanhui, Zhao
Yongkang, Ma
Shiming, Zhao
Zhenghui, Sun
Zheng, Du
Jiangshan, Peng
Tiejun, Yang
author_facet Huaqi, Yin
Bingqi, Dong
Yanhui, Zhao
Yongkang, Ma
Shiming, Zhao
Zhenghui, Sun
Zheng, Du
Jiangshan, Peng
Tiejun, Yang
author_sort Huaqi, Yin
collection PubMed
description BACKGROUND: In recent years, hyperthermia has been widely applied as a novel strategy for cancer treatment due to its multiple antitumour effects. In particular, the potential influences of hyperthermia on the tumour immune microenvironment may improve the efficacy of immunotherapies. However, the effect of hyperthermia on renal cell carcinoma (RCC) has not been well characterized until now. METHODS: In the present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration, invasion and apoptosis assays. In addition, the influence of hyperthermia on the immunogenicity of RCC cells was analysed using flow cytometry analysis, enzyme-linked immunosorbent assays, and immunofluorescent (IF) staining. RESULTS: Our results demonstrate that hyperthermia significantly inhibits RCC cell proliferation, migration, and invasion and promotes cell apoptosis. In addition, we verified that hyperthermia improves the immunogenicity of RCC cells by inducing immunogenic cell death. CONCLUSION: Our findings suggest that hyperthermia is a promising therapeutic strategy for RCC.
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spelling pubmed-105688112023-10-13 Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma Huaqi, Yin Bingqi, Dong Yanhui, Zhao Yongkang, Ma Shiming, Zhao Zhenghui, Sun Zheng, Du Jiangshan, Peng Tiejun, Yang BMC Cancer Research BACKGROUND: In recent years, hyperthermia has been widely applied as a novel strategy for cancer treatment due to its multiple antitumour effects. In particular, the potential influences of hyperthermia on the tumour immune microenvironment may improve the efficacy of immunotherapies. However, the effect of hyperthermia on renal cell carcinoma (RCC) has not been well characterized until now. METHODS: In the present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration, invasion and apoptosis assays. In addition, the influence of hyperthermia on the immunogenicity of RCC cells was analysed using flow cytometry analysis, enzyme-linked immunosorbent assays, and immunofluorescent (IF) staining. RESULTS: Our results demonstrate that hyperthermia significantly inhibits RCC cell proliferation, migration, and invasion and promotes cell apoptosis. In addition, we verified that hyperthermia improves the immunogenicity of RCC cells by inducing immunogenic cell death. CONCLUSION: Our findings suggest that hyperthermia is a promising therapeutic strategy for RCC. BioMed Central 2023-10-12 /pmc/articles/PMC10568811/ /pubmed/37828458 http://dx.doi.org/10.1186/s12885-023-11106-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Huaqi, Yin
Bingqi, Dong
Yanhui, Zhao
Yongkang, Ma
Shiming, Zhao
Zhenghui, Sun
Zheng, Du
Jiangshan, Peng
Tiejun, Yang
Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title_full Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title_fullStr Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title_full_unstemmed Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title_short Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
title_sort hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568811/
https://www.ncbi.nlm.nih.gov/pubmed/37828458
http://dx.doi.org/10.1186/s12885-023-11106-8
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