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Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species

BACKGROUND: Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease the capital and operational costs associated with scaled fermentation. The utilisation of the robust and halophilic industrial host organisms of the...

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Autores principales: Faulkner, Matthew, Hoeven, Robin, Kelly, Paul P., Sun, Yaqi, Park, Helen, Liu, Lu-Ning, Toogood, Helen S., Scrutton, Nigel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568851/
https://www.ncbi.nlm.nih.gov/pubmed/37821908
http://dx.doi.org/10.1186/s13068-023-02404-1
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author Faulkner, Matthew
Hoeven, Robin
Kelly, Paul P.
Sun, Yaqi
Park, Helen
Liu, Lu-Ning
Toogood, Helen S.
Scrutton, Nigel S.
author_facet Faulkner, Matthew
Hoeven, Robin
Kelly, Paul P.
Sun, Yaqi
Park, Helen
Liu, Lu-Ning
Toogood, Helen S.
Scrutton, Nigel S.
author_sort Faulkner, Matthew
collection PubMed
description BACKGROUND: Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease the capital and operational costs associated with scaled fermentation. The utilisation of the robust and halophilic industrial host organisms of the genus Halomonas could dramatically decrease biomanufacturing costs owing to their ability to grow in seawater, using waste biogenic feedstocks, under non-sterile conditions. RESULTS: We describe the isolation of Halomonas rowanensis, a novel facultative chemoautotrophic species of Halomonas from a natural brine spring. We investigated the ability of this species to produce ectoine, a compound of considerable industrial interest, under heterotrophic conditions. Fixation of radiolabelled NaH(14)CO(3) by H. rowanensis was confirmed in mineral medium supplied with thiosulfate as an energy source. Genome sequencing suggested carbon fixation proceeds via a reductive tricarboxylic acid cycle, and not the Calvin–Bensen–Bassham cycle. The mechanism of energy generation to support chemoautotrophy is unknown owing to the absence of an annotated SOX-based thiosulfate-mediated energy conversion system. We investigated further the biotechnological potential of the isolated H. rowanensis by demonstrating production of the gaseous hydrocarbon (bio-propane), bioplastics (poly-3-hydroxybutyrate) and osmolytes (ectoine) under heterotrophic and autotrophic CO(2) fixation growth conditions. CONCLUSIONS: This proof-of-concept study illustrates the value of recruiting environmental isolates as industrial hosts for chemicals biomanufacturing, where CO(2) utilisation could replace, or augment, the use of biogenic feedstocks in non-sterile, industrialised bioreactors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-023-02404-1.
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spelling pubmed-105688512023-10-13 Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species Faulkner, Matthew Hoeven, Robin Kelly, Paul P. Sun, Yaqi Park, Helen Liu, Lu-Ning Toogood, Helen S. Scrutton, Nigel S. Biotechnol Biofuels Bioprod Research BACKGROUND: Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease the capital and operational costs associated with scaled fermentation. The utilisation of the robust and halophilic industrial host organisms of the genus Halomonas could dramatically decrease biomanufacturing costs owing to their ability to grow in seawater, using waste biogenic feedstocks, under non-sterile conditions. RESULTS: We describe the isolation of Halomonas rowanensis, a novel facultative chemoautotrophic species of Halomonas from a natural brine spring. We investigated the ability of this species to produce ectoine, a compound of considerable industrial interest, under heterotrophic conditions. Fixation of radiolabelled NaH(14)CO(3) by H. rowanensis was confirmed in mineral medium supplied with thiosulfate as an energy source. Genome sequencing suggested carbon fixation proceeds via a reductive tricarboxylic acid cycle, and not the Calvin–Bensen–Bassham cycle. The mechanism of energy generation to support chemoautotrophy is unknown owing to the absence of an annotated SOX-based thiosulfate-mediated energy conversion system. We investigated further the biotechnological potential of the isolated H. rowanensis by demonstrating production of the gaseous hydrocarbon (bio-propane), bioplastics (poly-3-hydroxybutyrate) and osmolytes (ectoine) under heterotrophic and autotrophic CO(2) fixation growth conditions. CONCLUSIONS: This proof-of-concept study illustrates the value of recruiting environmental isolates as industrial hosts for chemicals biomanufacturing, where CO(2) utilisation could replace, or augment, the use of biogenic feedstocks in non-sterile, industrialised bioreactors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-023-02404-1. BioMed Central 2023-10-11 /pmc/articles/PMC10568851/ /pubmed/37821908 http://dx.doi.org/10.1186/s13068-023-02404-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Faulkner, Matthew
Hoeven, Robin
Kelly, Paul P.
Sun, Yaqi
Park, Helen
Liu, Lu-Ning
Toogood, Helen S.
Scrutton, Nigel S.
Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title_full Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title_fullStr Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title_full_unstemmed Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title_short Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species
title_sort chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel halomonas species
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568851/
https://www.ncbi.nlm.nih.gov/pubmed/37821908
http://dx.doi.org/10.1186/s13068-023-02404-1
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