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Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China

INTRODUCTION: The provincial malaria diagnosis reference laboratories review and assess malaria cases diagnosed in health facilities for supporting the malaria elimination efforts and preventing re-transmission of imported malaria. The study aimed to evaluate the detection capability of malaria diag...

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Autores principales: Zhang, Xuan, Jiang, Jingjing, Sui, Yuan, Yan, Hui, Xia, Jing, Liu, Ying, Sun, Lingcong, Wang, Xiaoxiao, Marfurt, Jutta, Lu, Shenning, Li, Shizhu, Ruan, Wei, Wang, Duoquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569120/
https://www.ncbi.nlm.nih.gov/pubmed/37841716
http://dx.doi.org/10.3389/fpubh.2023.1243642
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author Zhang, Xuan
Jiang, Jingjing
Sui, Yuan
Yan, Hui
Xia, Jing
Liu, Ying
Sun, Lingcong
Wang, Xiaoxiao
Marfurt, Jutta
Lu, Shenning
Li, Shizhu
Ruan, Wei
Wang, Duoquan
author_facet Zhang, Xuan
Jiang, Jingjing
Sui, Yuan
Yan, Hui
Xia, Jing
Liu, Ying
Sun, Lingcong
Wang, Xiaoxiao
Marfurt, Jutta
Lu, Shenning
Li, Shizhu
Ruan, Wei
Wang, Duoquan
author_sort Zhang, Xuan
collection PubMed
description INTRODUCTION: The provincial malaria diagnosis reference laboratories review and assess malaria cases diagnosed in health facilities for supporting the malaria elimination efforts and preventing re-transmission of imported malaria. The study aimed to evaluate the detection capability of malaria diagnosis in China from 2014 to 2021. METHODS: Data on malaria cases reported in the provincial-level administrative divisions (PLADs) of Anhui, Henan, Hubei, Guangxi, and Zhejiang from 2014 to 2021 were collected and analyzed. RESULTS: In total, 5,770 malaria cases were reported from 2014 to 2021, and 99.05% (5,715/5,770) were submitted to the provincial malaria diagnosis reference laboratories. The median time between malaria cases being reported and the samples being received by reference laboratories was 6 days (Interquartile range, IQR:3–12 days) from 2017 to 2021. Diagnosis of 5,680 samples in the laboratory were confirmed by provincial reference laboratories, including 3,970 cases of Plasmodium falciparum, 414 of P. vivax, 1,055 of P. ovale, 158 of P. malariae, 1 of P. knowlesi, and 82 of mixed infections. Plasmodium species of 5,141 confirmed cases were consistent with the initial diagnosis, with a species accuracy rate of 90.53% (5,141/5,679). The accuracy of P. falciparum diagnosis in health facilities was higher than that of non-falciparum species. The inconsistency between microscopy and nested polymerase chain reaction (nPCR) results of confirmatory diagnosis was mainly in malaria-positive versus malaria-negative cases, as well as in mixed versus single infection cases. CONCLUSION: The provincial malaria diagnosis reference laboratories have played an important role in ensuring the accuracy and reliability of Plasmodium diagnosis in health facilities. However, the results of this study imply that capacity training for the identification of Plasmodium species in health facilities is warranted.
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spelling pubmed-105691202023-10-13 Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China Zhang, Xuan Jiang, Jingjing Sui, Yuan Yan, Hui Xia, Jing Liu, Ying Sun, Lingcong Wang, Xiaoxiao Marfurt, Jutta Lu, Shenning Li, Shizhu Ruan, Wei Wang, Duoquan Front Public Health Public Health INTRODUCTION: The provincial malaria diagnosis reference laboratories review and assess malaria cases diagnosed in health facilities for supporting the malaria elimination efforts and preventing re-transmission of imported malaria. The study aimed to evaluate the detection capability of malaria diagnosis in China from 2014 to 2021. METHODS: Data on malaria cases reported in the provincial-level administrative divisions (PLADs) of Anhui, Henan, Hubei, Guangxi, and Zhejiang from 2014 to 2021 were collected and analyzed. RESULTS: In total, 5,770 malaria cases were reported from 2014 to 2021, and 99.05% (5,715/5,770) were submitted to the provincial malaria diagnosis reference laboratories. The median time between malaria cases being reported and the samples being received by reference laboratories was 6 days (Interquartile range, IQR:3–12 days) from 2017 to 2021. Diagnosis of 5,680 samples in the laboratory were confirmed by provincial reference laboratories, including 3,970 cases of Plasmodium falciparum, 414 of P. vivax, 1,055 of P. ovale, 158 of P. malariae, 1 of P. knowlesi, and 82 of mixed infections. Plasmodium species of 5,141 confirmed cases were consistent with the initial diagnosis, with a species accuracy rate of 90.53% (5,141/5,679). The accuracy of P. falciparum diagnosis in health facilities was higher than that of non-falciparum species. The inconsistency between microscopy and nested polymerase chain reaction (nPCR) results of confirmatory diagnosis was mainly in malaria-positive versus malaria-negative cases, as well as in mixed versus single infection cases. CONCLUSION: The provincial malaria diagnosis reference laboratories have played an important role in ensuring the accuracy and reliability of Plasmodium diagnosis in health facilities. However, the results of this study imply that capacity training for the identification of Plasmodium species in health facilities is warranted. Frontiers Media S.A. 2023-09-28 /pmc/articles/PMC10569120/ /pubmed/37841716 http://dx.doi.org/10.3389/fpubh.2023.1243642 Text en Copyright © 2023 Zhang, Jiang, Sui, Yan, Xia, Liu, Sun, Wang, Marfurt, Lu, Li, Ruan and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Zhang, Xuan
Jiang, Jingjing
Sui, Yuan
Yan, Hui
Xia, Jing
Liu, Ying
Sun, Lingcong
Wang, Xiaoxiao
Marfurt, Jutta
Lu, Shenning
Li, Shizhu
Ruan, Wei
Wang, Duoquan
Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title_full Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title_fullStr Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title_full_unstemmed Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title_short Evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of China
title_sort evaluation of performance for malaria diagnosis in health facilities by five provincial reference laboratories of china
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569120/
https://www.ncbi.nlm.nih.gov/pubmed/37841716
http://dx.doi.org/10.3389/fpubh.2023.1243642
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