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A cellular resolution atlas of Broca’s area
Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)–referenced system using innovative imaging and analy...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569704/ https://www.ncbi.nlm.nih.gov/pubmed/37824623 http://dx.doi.org/10.1126/sciadv.adg3844 |
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| author | Costantini, Irene Morgan, Leah Yang, Jiarui Balbastre, Yael Varadarajan, Divya Pesce, Luca Scardigli, Marina Mazzamuto, Giacomo Gavryusev, Vladislav Castelli, Filippo Maria Roffilli, Matteo Silvestri, Ludovico Laffey, Jessie Raia, Sophia Varghese, Merina Wicinski, Bridget Chang, Shuaibin Chen, Ichun Anderson Wang, Hui Cordero, Devani Vera, Matthew Nolan, Jackson Nestor, Kimberly Mora, Jocelyn Iglesias, Juan Eugenio Garcia Pallares, Erendira Evancic, Kathryn Augustinack, Jean C. Fogarty, Morgan Dalca, Adrian V. Frosch, Matthew P. Magnain, Caroline Frost, Robert van der Kouwe, Andre Chen, Shih-Chi Boas, David A. Pavone, Francesco Saverio Fischl, Bruce Hof, Patrick R. |
| author_facet | Costantini, Irene Morgan, Leah Yang, Jiarui Balbastre, Yael Varadarajan, Divya Pesce, Luca Scardigli, Marina Mazzamuto, Giacomo Gavryusev, Vladislav Castelli, Filippo Maria Roffilli, Matteo Silvestri, Ludovico Laffey, Jessie Raia, Sophia Varghese, Merina Wicinski, Bridget Chang, Shuaibin Chen, Ichun Anderson Wang, Hui Cordero, Devani Vera, Matthew Nolan, Jackson Nestor, Kimberly Mora, Jocelyn Iglesias, Juan Eugenio Garcia Pallares, Erendira Evancic, Kathryn Augustinack, Jean C. Fogarty, Morgan Dalca, Adrian V. Frosch, Matthew P. Magnain, Caroline Frost, Robert van der Kouwe, Andre Chen, Shih-Chi Boas, David A. Pavone, Francesco Saverio Fischl, Bruce Hof, Patrick R. |
| author_sort | Costantini, Irene |
| collection | PubMed |
| description | Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)–referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custom-made inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca’s area in a human postmortem specimen, within a whole-brain reference space atlas. |
| format | Online Article Text |
| id | pubmed-10569704 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | American Association for the Advancement of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-105697042023-10-13 A cellular resolution atlas of Broca’s area Costantini, Irene Morgan, Leah Yang, Jiarui Balbastre, Yael Varadarajan, Divya Pesce, Luca Scardigli, Marina Mazzamuto, Giacomo Gavryusev, Vladislav Castelli, Filippo Maria Roffilli, Matteo Silvestri, Ludovico Laffey, Jessie Raia, Sophia Varghese, Merina Wicinski, Bridget Chang, Shuaibin Chen, Ichun Anderson Wang, Hui Cordero, Devani Vera, Matthew Nolan, Jackson Nestor, Kimberly Mora, Jocelyn Iglesias, Juan Eugenio Garcia Pallares, Erendira Evancic, Kathryn Augustinack, Jean C. Fogarty, Morgan Dalca, Adrian V. Frosch, Matthew P. Magnain, Caroline Frost, Robert van der Kouwe, Andre Chen, Shih-Chi Boas, David A. Pavone, Francesco Saverio Fischl, Bruce Hof, Patrick R. Sci Adv Neuroscience Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)–referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custom-made inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca’s area in a human postmortem specimen, within a whole-brain reference space atlas. American Association for the Advancement of Science 2023-10-12 /pmc/articles/PMC10569704/ /pubmed/37824623 http://dx.doi.org/10.1126/sciadv.adg3844 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
| spellingShingle | Neuroscience Costantini, Irene Morgan, Leah Yang, Jiarui Balbastre, Yael Varadarajan, Divya Pesce, Luca Scardigli, Marina Mazzamuto, Giacomo Gavryusev, Vladislav Castelli, Filippo Maria Roffilli, Matteo Silvestri, Ludovico Laffey, Jessie Raia, Sophia Varghese, Merina Wicinski, Bridget Chang, Shuaibin Chen, Ichun Anderson Wang, Hui Cordero, Devani Vera, Matthew Nolan, Jackson Nestor, Kimberly Mora, Jocelyn Iglesias, Juan Eugenio Garcia Pallares, Erendira Evancic, Kathryn Augustinack, Jean C. Fogarty, Morgan Dalca, Adrian V. Frosch, Matthew P. Magnain, Caroline Frost, Robert van der Kouwe, Andre Chen, Shih-Chi Boas, David A. Pavone, Francesco Saverio Fischl, Bruce Hof, Patrick R. A cellular resolution atlas of Broca’s area |
| title | A cellular resolution atlas of Broca’s area |
| title_full | A cellular resolution atlas of Broca’s area |
| title_fullStr | A cellular resolution atlas of Broca’s area |
| title_full_unstemmed | A cellular resolution atlas of Broca’s area |
| title_short | A cellular resolution atlas of Broca’s area |
| title_sort | cellular resolution atlas of broca’s area |
| topic | Neuroscience |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569704/ https://www.ncbi.nlm.nih.gov/pubmed/37824623 http://dx.doi.org/10.1126/sciadv.adg3844 |
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