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First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody

With most of the T cells residing in the tissue, not the blood, developing noninvasive methods for in vivo quantification of their biodistribution and kinetics is important for studying their role in immune response and memory. This study presents the first use of dynamic positron emission tomograph...

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Autores principales: Omidvari, Negar, Jones, Terry, Price, Pat M., Ferre, April L., Lu, Jacqueline, Abdelhafez, Yasser G., Sen, Fatma, Cohen, Stuart H., Schmiedehausen, Kristin, Badawi, Ramsey D., Shacklett, Barbara L., Wilson, Ian, Cherry, Simon R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569706/
https://www.ncbi.nlm.nih.gov/pubmed/37824612
http://dx.doi.org/10.1126/sciadv.adh7968
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author Omidvari, Negar
Jones, Terry
Price, Pat M.
Ferre, April L.
Lu, Jacqueline
Abdelhafez, Yasser G.
Sen, Fatma
Cohen, Stuart H.
Schmiedehausen, Kristin
Badawi, Ramsey D.
Shacklett, Barbara L.
Wilson, Ian
Cherry, Simon R.
author_facet Omidvari, Negar
Jones, Terry
Price, Pat M.
Ferre, April L.
Lu, Jacqueline
Abdelhafez, Yasser G.
Sen, Fatma
Cohen, Stuart H.
Schmiedehausen, Kristin
Badawi, Ramsey D.
Shacklett, Barbara L.
Wilson, Ian
Cherry, Simon R.
author_sort Omidvari, Negar
collection PubMed
description With most of the T cells residing in the tissue, not the blood, developing noninvasive methods for in vivo quantification of their biodistribution and kinetics is important for studying their role in immune response and memory. This study presents the first use of dynamic positron emission tomography (PET) and kinetic modeling for in vivo measurement of CD8(+) T cell biodistribution in humans. A (89)Zr-labeled CD8-targeted minibody ((89)Zr-Df-Crefmirlimab) was used with total-body PET in healthy individuals (N = 3) and coronavirus disease 2019 (COVID-19) convalescent patients (N = 5). Kinetic modeling results aligned with T cell–trafficking effects expected in lymphoid organs. Tissue-to-blood ratios from the first 7 hours of imaging were higher in bone marrow of COVID-19 convalescent patients compared to controls, with an increasing trend between 2 and 6 months after infection, consistent with modeled net influx rates and peripheral blood flow cytometry analysis. These results provide a promising platform for using dynamic PET to study the total-body immune response and memory.
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spelling pubmed-105697062023-10-13 First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody Omidvari, Negar Jones, Terry Price, Pat M. Ferre, April L. Lu, Jacqueline Abdelhafez, Yasser G. Sen, Fatma Cohen, Stuart H. Schmiedehausen, Kristin Badawi, Ramsey D. Shacklett, Barbara L. Wilson, Ian Cherry, Simon R. Sci Adv Physical and Materials Sciences With most of the T cells residing in the tissue, not the blood, developing noninvasive methods for in vivo quantification of their biodistribution and kinetics is important for studying their role in immune response and memory. This study presents the first use of dynamic positron emission tomography (PET) and kinetic modeling for in vivo measurement of CD8(+) T cell biodistribution in humans. A (89)Zr-labeled CD8-targeted minibody ((89)Zr-Df-Crefmirlimab) was used with total-body PET in healthy individuals (N = 3) and coronavirus disease 2019 (COVID-19) convalescent patients (N = 5). Kinetic modeling results aligned with T cell–trafficking effects expected in lymphoid organs. Tissue-to-blood ratios from the first 7 hours of imaging were higher in bone marrow of COVID-19 convalescent patients compared to controls, with an increasing trend between 2 and 6 months after infection, consistent with modeled net influx rates and peripheral blood flow cytometry analysis. These results provide a promising platform for using dynamic PET to study the total-body immune response and memory. American Association for the Advancement of Science 2023-10-12 /pmc/articles/PMC10569706/ /pubmed/37824612 http://dx.doi.org/10.1126/sciadv.adh7968 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Omidvari, Negar
Jones, Terry
Price, Pat M.
Ferre, April L.
Lu, Jacqueline
Abdelhafez, Yasser G.
Sen, Fatma
Cohen, Stuart H.
Schmiedehausen, Kristin
Badawi, Ramsey D.
Shacklett, Barbara L.
Wilson, Ian
Cherry, Simon R.
First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title_full First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title_fullStr First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title_full_unstemmed First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title_short First-in-human immunoPET imaging of COVID-19 convalescent patients using dynamic total-body PET and a CD8-targeted minibody
title_sort first-in-human immunopet imaging of covid-19 convalescent patients using dynamic total-body pet and a cd8-targeted minibody
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569706/
https://www.ncbi.nlm.nih.gov/pubmed/37824612
http://dx.doi.org/10.1126/sciadv.adh7968
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