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A single-cell multi-omic atlas spanning the adult rhesus macaque brain

Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigen...

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Detalles Bibliográficos
Autores principales: Chiou, Kenneth L., Huang, Xingfan, Bohlen, Martin O., Tremblay, Sébastien, DeCasien, Alex R., O’Day, Diana R., Spurrell, Cailyn H., Gogate, Aishwarya A., Zintel, Trisha M., Andrews, Madeline G., Martínez, Melween I., Starita, Lea M., Montague, Michael J., Platt, Michael L., Shendure, Jay, Snyder-Mackler, Noah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569716/
https://www.ncbi.nlm.nih.gov/pubmed/37824616
http://dx.doi.org/10.1126/sciadv.adh1914
Descripción
Sumario:Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type–specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions.