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A single-cell multi-omic atlas spanning the adult rhesus macaque brain

Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigen...

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Autores principales: Chiou, Kenneth L., Huang, Xingfan, Bohlen, Martin O., Tremblay, Sébastien, DeCasien, Alex R., O’Day, Diana R., Spurrell, Cailyn H., Gogate, Aishwarya A., Zintel, Trisha M., Andrews, Madeline G., Martínez, Melween I., Starita, Lea M., Montague, Michael J., Platt, Michael L., Shendure, Jay, Snyder-Mackler, Noah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569716/
https://www.ncbi.nlm.nih.gov/pubmed/37824616
http://dx.doi.org/10.1126/sciadv.adh1914
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author Chiou, Kenneth L.
Huang, Xingfan
Bohlen, Martin O.
Tremblay, Sébastien
DeCasien, Alex R.
O’Day, Diana R.
Spurrell, Cailyn H.
Gogate, Aishwarya A.
Zintel, Trisha M.
Andrews, Madeline G.
Martínez, Melween I.
Starita, Lea M.
Montague, Michael J.
Platt, Michael L.
Shendure, Jay
Snyder-Mackler, Noah
author_facet Chiou, Kenneth L.
Huang, Xingfan
Bohlen, Martin O.
Tremblay, Sébastien
DeCasien, Alex R.
O’Day, Diana R.
Spurrell, Cailyn H.
Gogate, Aishwarya A.
Zintel, Trisha M.
Andrews, Madeline G.
Martínez, Melween I.
Starita, Lea M.
Montague, Michael J.
Platt, Michael L.
Shendure, Jay
Snyder-Mackler, Noah
author_sort Chiou, Kenneth L.
collection PubMed
description Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type–specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions.
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spelling pubmed-105697162023-10-13 A single-cell multi-omic atlas spanning the adult rhesus macaque brain Chiou, Kenneth L. Huang, Xingfan Bohlen, Martin O. Tremblay, Sébastien DeCasien, Alex R. O’Day, Diana R. Spurrell, Cailyn H. Gogate, Aishwarya A. Zintel, Trisha M. Andrews, Madeline G. Martínez, Melween I. Starita, Lea M. Montague, Michael J. Platt, Michael L. Shendure, Jay Snyder-Mackler, Noah Sci Adv Neuroscience Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type–specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions. American Association for the Advancement of Science 2023-10-12 /pmc/articles/PMC10569716/ /pubmed/37824616 http://dx.doi.org/10.1126/sciadv.adh1914 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Neuroscience
Chiou, Kenneth L.
Huang, Xingfan
Bohlen, Martin O.
Tremblay, Sébastien
DeCasien, Alex R.
O’Day, Diana R.
Spurrell, Cailyn H.
Gogate, Aishwarya A.
Zintel, Trisha M.
Andrews, Madeline G.
Martínez, Melween I.
Starita, Lea M.
Montague, Michael J.
Platt, Michael L.
Shendure, Jay
Snyder-Mackler, Noah
A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title_full A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title_fullStr A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title_full_unstemmed A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title_short A single-cell multi-omic atlas spanning the adult rhesus macaque brain
title_sort single-cell multi-omic atlas spanning the adult rhesus macaque brain
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569716/
https://www.ncbi.nlm.nih.gov/pubmed/37824616
http://dx.doi.org/10.1126/sciadv.adh1914
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