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Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate
Excessive light exposure can damage photoreceptors and lead to blindness. Oxidative stress serves a key role in photo-induced retinal damage. Free radical scavengers have been proven to protect against photo-damaged retinal degeneration. Fullerol, a potent antioxidant, has the potential to protect a...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570010/ https://www.ncbi.nlm.nih.gov/pubmed/37816275 http://dx.doi.org/10.1016/j.redox.2023.102911 |
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author | Cha, Zhe Yin, Zhiyuan A, Luodan Ge, Lingling Yang, Junling Huang, Xiaona Gao, Hui Chen, Xia Feng, Zhou Mo, Lingyue He, Juncai Zhu, Shuang Zhao, Maoru Tao, Zui Gu, Zhanjun Xu, Haiwei |
author_facet | Cha, Zhe Yin, Zhiyuan A, Luodan Ge, Lingling Yang, Junling Huang, Xiaona Gao, Hui Chen, Xia Feng, Zhou Mo, Lingyue He, Juncai Zhu, Shuang Zhao, Maoru Tao, Zui Gu, Zhanjun Xu, Haiwei |
author_sort | Cha, Zhe |
collection | PubMed |
description | Excessive light exposure can damage photoreceptors and lead to blindness. Oxidative stress serves a key role in photo-induced retinal damage. Free radical scavengers have been proven to protect against photo-damaged retinal degeneration. Fullerol, a potent antioxidant, has the potential to protect against ultraviolet-B (UVB)-induced cornea injury by activating the endogenous stem cells. However, its effects on cell fate determination of Müller glia (MG) between gliosis and de-differentiation remain unclear. Therefore, we established a MG lineage-tracing mouse model of light-induced retinal damage to examine the therapeutic effects of fullerol. Fullerol exhibited superior protection against light-induced retinal injury compared to glutathione (GSH) and reduced oxidative stress levels, inhibited gliosis by suppressing the TGF-β pathway, and enhanced the de-differentiation of MG cells. RNA sequencing revealed that transcription candidate pathways, including Nrf2 and Wnt10a pathways, were involved in fullerol-induced neuroprotection. Fullerol-mediated transcriptional changes were validated by qPCR, Western blotting, and immunostaining using mouse retinas and human-derived Müller cell lines MIO-M1 cells, confirming that fullerol possibly modulated the Nrf2, Wnt10a, and TGF-β pathways in MG, which suppressed gliosis and promoted the de-differentiation of MG in light-induced retinal degeneration, indicating its potential in treating retinal diseases. |
format | Online Article Text |
id | pubmed-10570010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105700102023-10-14 Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate Cha, Zhe Yin, Zhiyuan A, Luodan Ge, Lingling Yang, Junling Huang, Xiaona Gao, Hui Chen, Xia Feng, Zhou Mo, Lingyue He, Juncai Zhu, Shuang Zhao, Maoru Tao, Zui Gu, Zhanjun Xu, Haiwei Redox Biol Research Paper Excessive light exposure can damage photoreceptors and lead to blindness. Oxidative stress serves a key role in photo-induced retinal damage. Free radical scavengers have been proven to protect against photo-damaged retinal degeneration. Fullerol, a potent antioxidant, has the potential to protect against ultraviolet-B (UVB)-induced cornea injury by activating the endogenous stem cells. However, its effects on cell fate determination of Müller glia (MG) between gliosis and de-differentiation remain unclear. Therefore, we established a MG lineage-tracing mouse model of light-induced retinal damage to examine the therapeutic effects of fullerol. Fullerol exhibited superior protection against light-induced retinal injury compared to glutathione (GSH) and reduced oxidative stress levels, inhibited gliosis by suppressing the TGF-β pathway, and enhanced the de-differentiation of MG cells. RNA sequencing revealed that transcription candidate pathways, including Nrf2 and Wnt10a pathways, were involved in fullerol-induced neuroprotection. Fullerol-mediated transcriptional changes were validated by qPCR, Western blotting, and immunostaining using mouse retinas and human-derived Müller cell lines MIO-M1 cells, confirming that fullerol possibly modulated the Nrf2, Wnt10a, and TGF-β pathways in MG, which suppressed gliosis and promoted the de-differentiation of MG in light-induced retinal degeneration, indicating its potential in treating retinal diseases. Elsevier 2023-10-05 /pmc/articles/PMC10570010/ /pubmed/37816275 http://dx.doi.org/10.1016/j.redox.2023.102911 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Paper Cha, Zhe Yin, Zhiyuan A, Luodan Ge, Lingling Yang, Junling Huang, Xiaona Gao, Hui Chen, Xia Feng, Zhou Mo, Lingyue He, Juncai Zhu, Shuang Zhao, Maoru Tao, Zui Gu, Zhanjun Xu, Haiwei Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title | Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title_full | Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title_fullStr | Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title_full_unstemmed | Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title_short | Fullerol rescues the light-induced retinal damage by modulating Müller glia cell fate |
title_sort | fullerol rescues the light-induced retinal damage by modulating müller glia cell fate |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570010/ https://www.ncbi.nlm.nih.gov/pubmed/37816275 http://dx.doi.org/10.1016/j.redox.2023.102911 |
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