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Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration
Peripheral nerve injury is one of the more common forms of peripheral nerve disorders, and the most severe type of peripheral nerve injury is a defect with a gap. Biosynthetic cellulose membrane (BCM) is a commonly used material for repair and ligation of nerve defects with gaps. Meanwhile, exosomes...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570595/ https://www.ncbi.nlm.nih.gov/pubmed/37841087 http://dx.doi.org/10.1016/j.ibneur.2023.09.009 |
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author | Cui, Tian-Wei Lu, Li-Fang Cao, Xu-Dong Zhang, Quan-Peng He, Yue-Bin Wang, Ya-Ru Ren, Rui Ben, Xin-Yu Ni, Pan-Li Ma, Zhi-Jian Li, Yun-Qing Yi, Xi-Nan Feng, Ren-Jun |
author_facet | Cui, Tian-Wei Lu, Li-Fang Cao, Xu-Dong Zhang, Quan-Peng He, Yue-Bin Wang, Ya-Ru Ren, Rui Ben, Xin-Yu Ni, Pan-Li Ma, Zhi-Jian Li, Yun-Qing Yi, Xi-Nan Feng, Ren-Jun |
author_sort | Cui, Tian-Wei |
collection | PubMed |
description | Peripheral nerve injury is one of the more common forms of peripheral nerve disorders, and the most severe type of peripheral nerve injury is a defect with a gap. Biosynthetic cellulose membrane (BCM) is a commonly used material for repair and ligation of nerve defects with gaps. Meanwhile, exosomes from mesenchymal stem cells can promote cell growth and proliferation. We envision combining exosomes with BCMs to leverage the advantages of both to promote repair of peripheral nerve injury. Prepared exosomes were added to BCMs to form exosome-loaded BCMs (EXO-BCM) that were used for nerve repair in a rat model of sciatic nerve defects with gaps. We evaluated the repair activity using a pawprint experiment, measurement and statistical analyses of sciatica function index and thermal latency of paw withdrawal, and quantitation of the number and diameter of regenerated nerve fibers. Results indicated that EXO-BCM produced comprehensive and durable repair of peripheral nerve defects that were similar to those for autologous nerve transplantation, the gold standard for nerve defect repair. EXO-BCM is not predicted to cause donor site morbidity to the patient, in contrast to autologous nerve transplantation. Together these results indicate that an approach using EXO-BCM represents a promising alternative to autologous nerve transplantation, and could have broad applications for repair of nerve defects. |
format | Online Article Text |
id | pubmed-10570595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105705952023-10-14 Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration Cui, Tian-Wei Lu, Li-Fang Cao, Xu-Dong Zhang, Quan-Peng He, Yue-Bin Wang, Ya-Ru Ren, Rui Ben, Xin-Yu Ni, Pan-Li Ma, Zhi-Jian Li, Yun-Qing Yi, Xi-Nan Feng, Ren-Jun IBRO Neurosci Rep Research Paper Peripheral nerve injury is one of the more common forms of peripheral nerve disorders, and the most severe type of peripheral nerve injury is a defect with a gap. Biosynthetic cellulose membrane (BCM) is a commonly used material for repair and ligation of nerve defects with gaps. Meanwhile, exosomes from mesenchymal stem cells can promote cell growth and proliferation. We envision combining exosomes with BCMs to leverage the advantages of both to promote repair of peripheral nerve injury. Prepared exosomes were added to BCMs to form exosome-loaded BCMs (EXO-BCM) that were used for nerve repair in a rat model of sciatic nerve defects with gaps. We evaluated the repair activity using a pawprint experiment, measurement and statistical analyses of sciatica function index and thermal latency of paw withdrawal, and quantitation of the number and diameter of regenerated nerve fibers. Results indicated that EXO-BCM produced comprehensive and durable repair of peripheral nerve defects that were similar to those for autologous nerve transplantation, the gold standard for nerve defect repair. EXO-BCM is not predicted to cause donor site morbidity to the patient, in contrast to autologous nerve transplantation. Together these results indicate that an approach using EXO-BCM represents a promising alternative to autologous nerve transplantation, and could have broad applications for repair of nerve defects. Elsevier 2023-09-29 /pmc/articles/PMC10570595/ /pubmed/37841087 http://dx.doi.org/10.1016/j.ibneur.2023.09.009 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Cui, Tian-Wei Lu, Li-Fang Cao, Xu-Dong Zhang, Quan-Peng He, Yue-Bin Wang, Ya-Ru Ren, Rui Ben, Xin-Yu Ni, Pan-Li Ma, Zhi-Jian Li, Yun-Qing Yi, Xi-Nan Feng, Ren-Jun Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title | Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title_full | Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title_fullStr | Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title_full_unstemmed | Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title_short | Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
title_sort | exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570595/ https://www.ncbi.nlm.nih.gov/pubmed/37841087 http://dx.doi.org/10.1016/j.ibneur.2023.09.009 |
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