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Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers
This study purposed to investigate the alleviating effect of dietary curcumin supplementation on oxidative stress in the liver of broilers induced by diquat. One-day-old Cobb broilers (400) were selected and randomly divided into 5 groups, with 8 replicates and 10 broilers per replicate. The control...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571021/ https://www.ncbi.nlm.nih.gov/pubmed/37826902 http://dx.doi.org/10.1016/j.psj.2023.103132 |
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author | Wu, Fengyang Yang, Xinyu Wang, Fengxia Liu, Yanhua Han, Shuaijuan Liu, Shudong Zhang, Zhisheng Chen, Baojiang |
author_facet | Wu, Fengyang Yang, Xinyu Wang, Fengxia Liu, Yanhua Han, Shuaijuan Liu, Shudong Zhang, Zhisheng Chen, Baojiang |
author_sort | Wu, Fengyang |
collection | PubMed |
description | This study purposed to investigate the alleviating effect of dietary curcumin supplementation on oxidative stress in the liver of broilers induced by diquat. One-day-old Cobb broilers (400) were selected and randomly divided into 5 groups, with 8 replicates and 10 broilers per replicate. The control group and the diquat group were fed the basal diet, while the curcumin supplementation groups were fed the basal diet supplemented with different amounts of curcumin (50, 100, and 150 mg/kg). On d 21 of the test, 1 broiler was randomly selected from each replicate and intraperitoneally injected with 20 mg/mL of diquat solution at a dose of 1 mL/kg BW or equivalent physiological saline (for the control group). After 48 h of feeding, the selected broilers were slaughtered for analysis. The results show that diquat treatment reduced the antioxidant capacity of the liver, caused oxidative stress, and affected its lipid metabolism. However, diet supplementation using curcumin completely or partially reversed the effect of diquat on the liver of broilers. The blood alanine aminotransferase activity, total bilirubin and total protein levels, and liver Caspase-3 mRNA abundance in broilers were lower or significantly lower in the curcumin supplementation group than in the diquat group (P < 0.05). The curcumin supplementation groups had significantly higher total antioxidant capacity activity but significantly lower malondialdehyde in the liver of broilers than the diquat group (P < 0.05). The blood triglyceride level of broilers was lower or significantly lower in the curcumin supplementation groups than in the diquat group (P < 0.05). The activities of cetyl coenzyme A carboxylase in the liver were significantly lower in the 150 mg/kg curcumin supplementation groups than in the DQ group (P < 0.05). In conclusion, dietary curcumin supplementation could ameliorate the effects of diquat-induced oxidative stress on the antioxidant capacity, tissue morphology, and lipid metabolism of the liver of broilers, thus protecting the liver. The recommended dosage for broiler diets is 100 to 150 mg/kg curcumin. |
format | Online Article Text |
id | pubmed-10571021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105710212023-10-14 Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers Wu, Fengyang Yang, Xinyu Wang, Fengxia Liu, Yanhua Han, Shuaijuan Liu, Shudong Zhang, Zhisheng Chen, Baojiang Poult Sci METABOLISM AND NUTRITION This study purposed to investigate the alleviating effect of dietary curcumin supplementation on oxidative stress in the liver of broilers induced by diquat. One-day-old Cobb broilers (400) were selected and randomly divided into 5 groups, with 8 replicates and 10 broilers per replicate. The control group and the diquat group were fed the basal diet, while the curcumin supplementation groups were fed the basal diet supplemented with different amounts of curcumin (50, 100, and 150 mg/kg). On d 21 of the test, 1 broiler was randomly selected from each replicate and intraperitoneally injected with 20 mg/mL of diquat solution at a dose of 1 mL/kg BW or equivalent physiological saline (for the control group). After 48 h of feeding, the selected broilers were slaughtered for analysis. The results show that diquat treatment reduced the antioxidant capacity of the liver, caused oxidative stress, and affected its lipid metabolism. However, diet supplementation using curcumin completely or partially reversed the effect of diquat on the liver of broilers. The blood alanine aminotransferase activity, total bilirubin and total protein levels, and liver Caspase-3 mRNA abundance in broilers were lower or significantly lower in the curcumin supplementation group than in the diquat group (P < 0.05). The curcumin supplementation groups had significantly higher total antioxidant capacity activity but significantly lower malondialdehyde in the liver of broilers than the diquat group (P < 0.05). The blood triglyceride level of broilers was lower or significantly lower in the curcumin supplementation groups than in the diquat group (P < 0.05). The activities of cetyl coenzyme A carboxylase in the liver were significantly lower in the 150 mg/kg curcumin supplementation groups than in the DQ group (P < 0.05). In conclusion, dietary curcumin supplementation could ameliorate the effects of diquat-induced oxidative stress on the antioxidant capacity, tissue morphology, and lipid metabolism of the liver of broilers, thus protecting the liver. The recommended dosage for broiler diets is 100 to 150 mg/kg curcumin. Elsevier 2023-09-20 /pmc/articles/PMC10571021/ /pubmed/37826902 http://dx.doi.org/10.1016/j.psj.2023.103132 Text en © 2023 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | METABOLISM AND NUTRITION Wu, Fengyang Yang, Xinyu Wang, Fengxia Liu, Yanhua Han, Shuaijuan Liu, Shudong Zhang, Zhisheng Chen, Baojiang Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title | Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title_full | Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title_fullStr | Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title_full_unstemmed | Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title_short | Dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
title_sort | dietary curcumin supplementation alleviates diquat-induced oxidative stress in the liver of broilers |
topic | METABOLISM AND NUTRITION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571021/ https://www.ncbi.nlm.nih.gov/pubmed/37826902 http://dx.doi.org/10.1016/j.psj.2023.103132 |
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