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Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat

BACKGROUND: Endophytic bacteria provide nutrients and stimulate systemic resistance during seed germination and plant growth and development, and their functional properties in combating various stresses make them a powerful tool in green agricultural production. In this paper we explored the functi...

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Autores principales: Qu, Hao, Wang, Yi, Wang, Baijuan, Li, Chengyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571398/
https://www.ncbi.nlm.nih.gov/pubmed/37833633
http://dx.doi.org/10.1186/s12866-023-02943-5
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author Qu, Hao
Wang, Yi
Wang, Baijuan
Li, Chengyun
author_facet Qu, Hao
Wang, Yi
Wang, Baijuan
Li, Chengyun
author_sort Qu, Hao
collection PubMed
description BACKGROUND: Endophytic bacteria provide nutrients and stimulate systemic resistance during seed germination and plant growth and development, and their functional properties in combating various stresses make them a powerful tool in green agricultural production. In this paper we explored the function of the endophyte community in buckwheat seeds in order to provide a theoretical basis for the application and scientific research of endophytes in buckwheat cultivation. We used pulsed electric field (PEF) technology to treat buckwheat seeds, monitored the effect of high-voltage pulse treatment on buckwheat seed germination, and analyzed the diversity of endophytic bacteria in buckwheat seeds using the amplicon sequencing method. RESULTS: PEF treatment promoted root development during buckwheat seed germination. A total of 350 Operational taxonomic units (OTUs) that were assigned into 103 genera were obtained from control and treatment groups using 16SrRNA amplicon sequencing technology. Additionally, PEF treatment also caused a significant decrease in the abundance of Actinobacteria, Proteobacteria, and Bacteroidetes. The abundance of 28 genera changed significantly as well: 11 genera were more abundant, and 17 were less abundant. The number of associated network edges was reduced from 980 to 117, the number of positive correlations decreased by 89.1%, and the number of negative correlations decreased by 86.6%. CONCLUSION: PEF treatment promoted early root development in buckwheat and was able to alter the seed endophytic bacterial community. This study thus makes a significant contribution to the field of endophyte research and to the application of PEF technology in plant cultivation.
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spelling pubmed-105713982023-10-14 Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat Qu, Hao Wang, Yi Wang, Baijuan Li, Chengyun BMC Microbiol Research BACKGROUND: Endophytic bacteria provide nutrients and stimulate systemic resistance during seed germination and plant growth and development, and their functional properties in combating various stresses make them a powerful tool in green agricultural production. In this paper we explored the function of the endophyte community in buckwheat seeds in order to provide a theoretical basis for the application and scientific research of endophytes in buckwheat cultivation. We used pulsed electric field (PEF) technology to treat buckwheat seeds, monitored the effect of high-voltage pulse treatment on buckwheat seed germination, and analyzed the diversity of endophytic bacteria in buckwheat seeds using the amplicon sequencing method. RESULTS: PEF treatment promoted root development during buckwheat seed germination. A total of 350 Operational taxonomic units (OTUs) that were assigned into 103 genera were obtained from control and treatment groups using 16SrRNA amplicon sequencing technology. Additionally, PEF treatment also caused a significant decrease in the abundance of Actinobacteria, Proteobacteria, and Bacteroidetes. The abundance of 28 genera changed significantly as well: 11 genera were more abundant, and 17 were less abundant. The number of associated network edges was reduced from 980 to 117, the number of positive correlations decreased by 89.1%, and the number of negative correlations decreased by 86.6%. CONCLUSION: PEF treatment promoted early root development in buckwheat and was able to alter the seed endophytic bacterial community. This study thus makes a significant contribution to the field of endophyte research and to the application of PEF technology in plant cultivation. BioMed Central 2023-10-13 /pmc/articles/PMC10571398/ /pubmed/37833633 http://dx.doi.org/10.1186/s12866-023-02943-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Qu, Hao
Wang, Yi
Wang, Baijuan
Li, Chengyun
Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title_full Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title_fullStr Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title_full_unstemmed Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title_short Pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
title_sort pulsed electric field treatment of seeds altered the endophytic bacterial community and promotes early growth of roots in buckwheat
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571398/
https://www.ncbi.nlm.nih.gov/pubmed/37833633
http://dx.doi.org/10.1186/s12866-023-02943-5
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