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World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses

SIMPLE SUMMARY: Although many new cancer drugs look like they are working in the laboratory, they fail when tested on real people. This is often because the cancer cell models they are tested on are short-term, but cancer grows in people over a longer period, and things change over time. This is not...

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Autores principales: McGuckin, Colin, Forraz, Nico, Milet, Clément, Lacroix, Mathieu, Sbirkov, Yordan, Sarafian, Victoria, Ebel, Caroline, Spindler, Anita, Koerper, Véronique, Balloul, Jean-Marc, Quéméneur, Eric, Zaupa, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571882/
https://www.ncbi.nlm.nih.gov/pubmed/37835418
http://dx.doi.org/10.3390/cancers15194724
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author McGuckin, Colin
Forraz, Nico
Milet, Clément
Lacroix, Mathieu
Sbirkov, Yordan
Sarafian, Victoria
Ebel, Caroline
Spindler, Anita
Koerper, Véronique
Balloul, Jean-Marc
Quéméneur, Eric
Zaupa, Cécile
author_facet McGuckin, Colin
Forraz, Nico
Milet, Clément
Lacroix, Mathieu
Sbirkov, Yordan
Sarafian, Victoria
Ebel, Caroline
Spindler, Anita
Koerper, Véronique
Balloul, Jean-Marc
Quéméneur, Eric
Zaupa, Cécile
author_sort McGuckin, Colin
collection PubMed
description SIMPLE SUMMARY: Although many new cancer drugs look like they are working in the laboratory, they fail when tested on real people. This is often because the cancer cell models they are tested on are short-term, but cancer grows in people over a longer period, and things change over time. This is not a criticism of cancer laboratories, but more a realism of the limits of scientific research. This is why we developed this longer-lasting model of colorectal cancer that has moved around the body. At the same time, we used this model to test next-generation delivery of chemotherapy drugs to tumors with an advanced targeting virus. All levels of cancer research have value, both short-term and long-term, but the longer strategies need to catch up, so this study demonstrates what can be achieved with 3D bioprinting tumor models and testing viruses transformed for good. ABSTRACT: Long-term modelization of cancer as it changes in the human body is a difficult goal, particularly when designing and testing new therapeutic strategies. This becomes even more difficult with metastasis modeling to show chemotherapeutic molecule delivery directly to tumoral cells. Advanced therapeutics, including oncolytic viruses, antibody-based and cell-based therapies are increasing. The question is, are screening tests also evolving? Next-generation therapeutics need equally advanced screening tests, which whilst difficult to achieve, are the goal of our work here, creating models of micro- and macrotumors using 3D bioprinting. We developed advanced colorectal cancer tumor processing techniques to provide options for cellular expansion, microtumor printing, and long-term models, which allow for the evaluation of the kinetics of penetration testing, therapeutic success, targeted therapies, and personalized medicine. We describe how we tested tumors from a primary colorectal patient and, applying 3D bioprinting, matured long-term models for oncolytic metastatic screening. Three-dimensional microtumors were kept alive for the longest time ever recorded in vitro, allowing longitudinal studies, screening of oncolytic viruses and realistic modelization of colorectal cancer. These 3D bioprinted models were maintained for around 6 months and were able to demonstrate the effective delivery of a product to the tumoral environment and represent a step forward in therapeutic screening.
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spelling pubmed-105718822023-10-14 World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses McGuckin, Colin Forraz, Nico Milet, Clément Lacroix, Mathieu Sbirkov, Yordan Sarafian, Victoria Ebel, Caroline Spindler, Anita Koerper, Véronique Balloul, Jean-Marc Quéméneur, Eric Zaupa, Cécile Cancers (Basel) Article SIMPLE SUMMARY: Although many new cancer drugs look like they are working in the laboratory, they fail when tested on real people. This is often because the cancer cell models they are tested on are short-term, but cancer grows in people over a longer period, and things change over time. This is not a criticism of cancer laboratories, but more a realism of the limits of scientific research. This is why we developed this longer-lasting model of colorectal cancer that has moved around the body. At the same time, we used this model to test next-generation delivery of chemotherapy drugs to tumors with an advanced targeting virus. All levels of cancer research have value, both short-term and long-term, but the longer strategies need to catch up, so this study demonstrates what can be achieved with 3D bioprinting tumor models and testing viruses transformed for good. ABSTRACT: Long-term modelization of cancer as it changes in the human body is a difficult goal, particularly when designing and testing new therapeutic strategies. This becomes even more difficult with metastasis modeling to show chemotherapeutic molecule delivery directly to tumoral cells. Advanced therapeutics, including oncolytic viruses, antibody-based and cell-based therapies are increasing. The question is, are screening tests also evolving? Next-generation therapeutics need equally advanced screening tests, which whilst difficult to achieve, are the goal of our work here, creating models of micro- and macrotumors using 3D bioprinting. We developed advanced colorectal cancer tumor processing techniques to provide options for cellular expansion, microtumor printing, and long-term models, which allow for the evaluation of the kinetics of penetration testing, therapeutic success, targeted therapies, and personalized medicine. We describe how we tested tumors from a primary colorectal patient and, applying 3D bioprinting, matured long-term models for oncolytic metastatic screening. Three-dimensional microtumors were kept alive for the longest time ever recorded in vitro, allowing longitudinal studies, screening of oncolytic viruses and realistic modelization of colorectal cancer. These 3D bioprinted models were maintained for around 6 months and were able to demonstrate the effective delivery of a product to the tumoral environment and represent a step forward in therapeutic screening. MDPI 2023-09-26 /pmc/articles/PMC10571882/ /pubmed/37835418 http://dx.doi.org/10.3390/cancers15194724 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
McGuckin, Colin
Forraz, Nico
Milet, Clément
Lacroix, Mathieu
Sbirkov, Yordan
Sarafian, Victoria
Ebel, Caroline
Spindler, Anita
Koerper, Véronique
Balloul, Jean-Marc
Quéméneur, Eric
Zaupa, Cécile
World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title_full World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title_fullStr World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title_full_unstemmed World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title_short World’s First Long-Term Colorectal Cancer Model by 3D Bioprinting as a Mechanism for Screening Oncolytic Viruses
title_sort world’s first long-term colorectal cancer model by 3d bioprinting as a mechanism for screening oncolytic viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571882/
https://www.ncbi.nlm.nih.gov/pubmed/37835418
http://dx.doi.org/10.3390/cancers15194724
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