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Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method

Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we r...

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Autores principales: Starodubtseva, Maria N., Shkliarava, Nastassia M., Chelnokova, Irina A., Villalba, María I., Krylov, Andrei Yu., Nadyrov, Eldar A., Kasas, Sandor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572077/
https://www.ncbi.nlm.nih.gov/pubmed/37830577
http://dx.doi.org/10.3390/cells12192362
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author Starodubtseva, Maria N.
Shkliarava, Nastassia M.
Chelnokova, Irina A.
Villalba, María I.
Krylov, Andrei Yu.
Nadyrov, Eldar A.
Kasas, Sandor
author_facet Starodubtseva, Maria N.
Shkliarava, Nastassia M.
Chelnokova, Irina A.
Villalba, María I.
Krylov, Andrei Yu.
Nadyrov, Eldar A.
Kasas, Sandor
author_sort Starodubtseva, Maria N.
collection PubMed
description Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we revealed the unique patterns of the dependence of Young’s modulus on the indentation depth for two cancer cell lines that correlate with the features of the spatial organization of the actin cytoskeleton. Within a 200–300 nm layer just under the cell membrane, BT-20 cells are stiffer than ZR-75 cells, whereas in deeper cell regions, Young’s modulus of ZR-75 cells exceeds that of BT-20 cells. Two cancer cell lines also displayed a difference in cell nanomotion dynamics upon exposure to cytochalasin D, a potent actin polymerization inhibitor. The drug strongly modified the nanomotion pattern of BT-20 cells, whereas it had almost no effect on the ZR-75 cells. We are confident that nanomotion monitoring and measurement of the stiffness of cancer cells at various indentation depths deserve further studies to obtain effective predictive parameters for use in clinical practice.
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spelling pubmed-105720772023-10-14 Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method Starodubtseva, Maria N. Shkliarava, Nastassia M. Chelnokova, Irina A. Villalba, María I. Krylov, Andrei Yu. Nadyrov, Eldar A. Kasas, Sandor Cells Article Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we revealed the unique patterns of the dependence of Young’s modulus on the indentation depth for two cancer cell lines that correlate with the features of the spatial organization of the actin cytoskeleton. Within a 200–300 nm layer just under the cell membrane, BT-20 cells are stiffer than ZR-75 cells, whereas in deeper cell regions, Young’s modulus of ZR-75 cells exceeds that of BT-20 cells. Two cancer cell lines also displayed a difference in cell nanomotion dynamics upon exposure to cytochalasin D, a potent actin polymerization inhibitor. The drug strongly modified the nanomotion pattern of BT-20 cells, whereas it had almost no effect on the ZR-75 cells. We are confident that nanomotion monitoring and measurement of the stiffness of cancer cells at various indentation depths deserve further studies to obtain effective predictive parameters for use in clinical practice. MDPI 2023-09-26 /pmc/articles/PMC10572077/ /pubmed/37830577 http://dx.doi.org/10.3390/cells12192362 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Starodubtseva, Maria N.
Shkliarava, Nastassia M.
Chelnokova, Irina A.
Villalba, María I.
Krylov, Andrei Yu.
Nadyrov, Eldar A.
Kasas, Sandor
Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title_full Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title_fullStr Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title_full_unstemmed Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title_short Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
title_sort mechanical properties and nanomotion of bt-20 and zr-75 breast cancer cells studied by atomic force microscopy and optical nanomotion detection method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572077/
https://www.ncbi.nlm.nih.gov/pubmed/37830577
http://dx.doi.org/10.3390/cells12192362
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