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Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics

Salt stress severely restricts plant growth and crop production, which is accompanied by accumulation of reactive oxygen species (ROS) that disturb cell redox homeostasis and oxidize redox-sensitive proteins. Eutrema salsugineum, a halophytic species closely related to Arabidopsis, shows a high leve...

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Autores principales: Li, Jiawen, Yang, Xiaomin, Liu, Fuqing, Liu, Xinxin, Zhao, Tong, Yan, Xiufeng, Pang, Qiuying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572166/
https://www.ncbi.nlm.nih.gov/pubmed/37833966
http://dx.doi.org/10.3390/ijms241914518
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author Li, Jiawen
Yang, Xiaomin
Liu, Fuqing
Liu, Xinxin
Zhao, Tong
Yan, Xiufeng
Pang, Qiuying
author_facet Li, Jiawen
Yang, Xiaomin
Liu, Fuqing
Liu, Xinxin
Zhao, Tong
Yan, Xiufeng
Pang, Qiuying
author_sort Li, Jiawen
collection PubMed
description Salt stress severely restricts plant growth and crop production, which is accompanied by accumulation of reactive oxygen species (ROS) that disturb cell redox homeostasis and oxidize redox-sensitive proteins. Eutrema salsugineum, a halophytic species closely related to Arabidopsis, shows a high level of tolerance to salinity and is increasingly used as a model plant in abiotic stress biology. To understand redox modifications and signaling pathways under salt stress, we used tandem mass tag (TMT)-based proteomics to quantify the salt-induced changes in protein redox modifications in E. salsugineum. Salt stress led to increased oxidative modification levels of 159 cysteine sites in 107 proteins, which play roles in carbohydrate and energy metabolism, transport, ROS homeostasis, cellular structure modulation, and folding and assembly. These lists of unknown redox reactive proteins in salt mustard lay the foundation for future research to understand the molecular mechanism of plant salt response. However, glutathione peroxidase (GPX) is one of the most important antioxidant enzymes in plants. Our research indicates that EsGPX may be involved in regulating ROS levels and that plants with overexpressed EsGPX have much improved salt tolerance.
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spelling pubmed-105721662023-10-14 Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics Li, Jiawen Yang, Xiaomin Liu, Fuqing Liu, Xinxin Zhao, Tong Yan, Xiufeng Pang, Qiuying Int J Mol Sci Article Salt stress severely restricts plant growth and crop production, which is accompanied by accumulation of reactive oxygen species (ROS) that disturb cell redox homeostasis and oxidize redox-sensitive proteins. Eutrema salsugineum, a halophytic species closely related to Arabidopsis, shows a high level of tolerance to salinity and is increasingly used as a model plant in abiotic stress biology. To understand redox modifications and signaling pathways under salt stress, we used tandem mass tag (TMT)-based proteomics to quantify the salt-induced changes in protein redox modifications in E. salsugineum. Salt stress led to increased oxidative modification levels of 159 cysteine sites in 107 proteins, which play roles in carbohydrate and energy metabolism, transport, ROS homeostasis, cellular structure modulation, and folding and assembly. These lists of unknown redox reactive proteins in salt mustard lay the foundation for future research to understand the molecular mechanism of plant salt response. However, glutathione peroxidase (GPX) is one of the most important antioxidant enzymes in plants. Our research indicates that EsGPX may be involved in regulating ROS levels and that plants with overexpressed EsGPX have much improved salt tolerance. MDPI 2023-09-25 /pmc/articles/PMC10572166/ /pubmed/37833966 http://dx.doi.org/10.3390/ijms241914518 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jiawen
Yang, Xiaomin
Liu, Fuqing
Liu, Xinxin
Zhao, Tong
Yan, Xiufeng
Pang, Qiuying
Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title_full Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title_fullStr Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title_full_unstemmed Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title_short Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics
title_sort redox regulation of salt tolerance in eutrema salsugineum by proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572166/
https://www.ncbi.nlm.nih.gov/pubmed/37833966
http://dx.doi.org/10.3390/ijms241914518
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